| 王勇,马武华,罗艳霞,邓恋,蔡诚毅.川芎嗪对自体血回输患者T淋巴细胞免疫功能的影响[J].,2011,31(2):188-190,203 |
| 川芎嗪对自体血回输患者T淋巴细胞免疫功能的影响 |
| Effect of Ligustrazine on T-lymphocyte Immunity in Patients Undergoing Autologous Blood Transfusion |
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| DOI： |
| 中文关键词: 自体血回输 川芎嗪 T淋巴细胞 免疫功能 |
| 英文关键词:autologous blood transfusion ligustrazine T-lymphocyte immunity |
| 基金项目:广东省科学技术厅社会发展计划项目(No.2009B030801303);广东省中医药局建设中医药强省科研课题基金资助项目(No.2009397);2008年度贝朗麻醉科学研究基金资助项目 |
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| 中文摘要: |
| 目的探讨川芎嗪对自体血回输患者T淋巴细胞免疫功能的保护作用。方法 40例择期行脊柱手术患者,美国麻醉医师协会(American Society of Anesthesiologis,t ASA)分级Ⅰ～Ⅱ级,术前评估出血量>400mL,无血液及内分泌等系统疾病,随机分成试验组和对照组,每组20例。试验组于术前首次采血后,在收集自体血前30min经静脉滴注川芎嗪2mg/kg,在回收血液的肝素盐水和洗涤盐水内加入川芎嗪,终浓度为0.005%;对照组始终不予川芎嗪,余同试验组。记录术中出血量及回输血量,采集术前、回输自体血后1h、1d、5d的静脉血,使用流式细胞仪测定T淋巴细胞亚群(CD3、CD4、CD8)的水平,并计算CD4/CD8值。结果两组CD3水平在术后1h、1d较术前均明显下降(P<0.05,P<0.01),术后5d对照组较术前仍低(P<0.05),而试验组已恢复至术前水平;在术后各时间点,对照组CD3水平均明显低于试验组(P<0.05,P<0.01)。对照组CD4在术后1h、1d较术前明显下降(P<0.01),至术后5d才基本恢复至术前水平;试验组CD4在回输血后1h较术前明显下降(P<0.01),但术后1d已恢复至术前水平;术后1h、1d对照组CD4水平均低于试验组(P<0.05,P<0.01)。对照组CD4/CD8值在术后1d、5d较术前明显下降,且低于试验组,差异均有统计学意义(P<0.05)。结论试验组T淋巴细胞免疫功能的抑制程度明显较对照组轻,且恢复时间明显比对照组快,表明川芎嗪在自体血回输中对T淋巴细胞免疫功能具有一定的保护作用。 |
| 英文摘要: |
| Objective To evaluate the protective effect of ligustrazine on T-lymphocyte immunity of patients undergoing autologous blood transfusion (ABT). Methods Forty American Society of Anesthesiologist (ASA) Ⅰ-Ⅱ patients scheduled for receiving selective lumbar surgery,with pre-operational anticipatory blood loss>400 mL and without any diseases of blood or endocrinal system were enrolled. They were equally randomized into two groups,the trial group and the control group. Ligustrazine was given to patients in the trial group by intravenous dripping at the dose of 2 mg/kg,30 min before auto-blood collection,also by mixed in the washing saline and heparinized saline solution to make the final concentration of ligustrazine 0.005%. No ligustrazine was given to patients in the control group. The amount of blood loss and autotransfused were measured and recorded; and patients’ venous blood samples for T-lymphocyte subsets (CD3,CD4,CD8) determination and CD4/CD8 ratio calculation were collected at different time points,i.e. before surgery (T0) and at 1 h (T1),1st day (T2) and 5th day (T3) after ABT. Results CD3 decreased obviously at T1 and T2 in both groups (P<0.05 or P<0.01),while at T3,it restored to baseline in the trial group,but remained at the low level in the control group (P<0.05). Moreover,levels of CD3 at T1-T3 were lower in the control group than those in the trial group respectively (P<0.05 or P<0.01). CD4 decreased obviously at T1 (P<0.01) in both groups,it recovered at T2 in the trial group,but the recovering in the control group was T3,so comparison of CD4 level between groupsshowed significant difference at T1 and T2 (P<0.05 or P<0.01). As for CD4/CD8 ratio,it decreased obviously at T2 and T3 in the control group (P<0.05),but unchanged in the trial group,showing statistical difference between groups (P<0.05). Conclusion Ligustrazine had definite protective effect on T-lymphocyte immunity in patients undergoing ABT,which was presented by the milder inhibition and quicker recovery of immunity. |
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