| 周荣耀,钟薏,赵喜,张安君,罗春蕾,刘慧.补肾健脾方及其拆方对移植性原发性肝癌小鼠肿瘤生长的影响及差异研究[J].,2011,31(2):213-217 |
| 补肾健脾方及其拆方对移植性原发性肝癌小鼠肿瘤生长的影响及差异研究 |
| Effect of Bushen Jianpi Decoction and Its Disassemble Recipes on Tumor Growth in Mice with Transplanted Primary Hepatic Carcinoma |
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| DOI： |
| 中文关键词: 补肾健脾方 拆方 表观扩散系数 血管内皮生长因子 细胞凋亡 |
| 英文关键词:Bushen Jianpi Decoction the disassemble recipe apparent diffusion coefficient vascular endothelial growth factor cell apoptosis |
| 基金项目:国家自然科学基金重大研究计划资助项目(No.90209033);上海市教委课题(No.07CZ022) |
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| 中文摘要: |
| 目的探讨补肾健脾方及其拆方对移植性原发性肝癌小鼠肿瘤生长的影响及差异。方法 50只荷移植性原发性肝癌小鼠随机分为模型组、补肾健脾组、补肾组、健脾组及5-FU(5-氟尿嘧啶)组,每组10只。模型组和各中药组小鼠分别给予蒸馏水和相应中药方灌胃,每天1次,连续给药10天;5-FU组隔天给予5-FU腹腔注射。观察各组小鼠的体重、瘤重、表观扩散系数(apparentdiffusion coefficien,t ADC)、血清血管内皮生长因子(vascular endothelial growth factor,VEGF)、肿瘤细胞凋亡及细胞周期。结果各治疗组中,小鼠体重和去瘤体重比较,补肾健脾组高于其他各组(P<0.01,P<0.05);肿瘤指数比较,补肾健脾组低于其他各组(P<0.05);肿瘤ADC比较,补肾健脾组、5-FU组均低于补肾组和健脾组(P<0.05);血清VEGF及肿瘤瘤重比较,5-FU组低于各中药组(P<0.01,P<0.05),补肾组、补肾健脾组低于健脾组(P<0.05);抑瘤率及细胞凋亡比较,5-FU组最高,补肾健脾组次之;各组S期细胞比较,5-FU组低于各中药组(P<0.01,P<0.05),补肾健脾组显著低于补肾组和健脾组(P<0.01);G0-G1期比较,健脾组低于5-FU组(P<0.01),高于补肾组和补肾健脾组(P<0.05)。G2-M期各组比较差异无统计学意义。结论补肾健脾方及其拆方对小鼠移植性原发性肝癌的作用机制在于抑制血清VEGF的表达,促进肿瘤细胞的凋亡;MRI弥散技术ADC值的变化与VEGF和细胞凋亡同步,能客观反应肿瘤细胞代谢,是评定中药疗效的新型手段。 |
| 英文摘要: |
| Objective To observe comparatively the effects of Bushen Jianpi Decoction (BJD) and its disassemble recipes on tumor growth in mice with transplanted primary hepatic carcinoma (PCH). Methods Fifty mice with transplanted PCH were randomly and equally divided into 5 groups. The blank control group consisted of untreated model mice,and 4 treated groups consisted of model mice treated with 5-FU (q.o.d,intraperitoneal injection,×5),BJD and it disassemble recipes (Bushen portion and Jianpi portion,abbreviated as BSP and JPP hereafter) q.d. by gastrogavage for 10 successive days. The body mass,tumor size,serum vascular endothelial growth factor (VEGF) level,tumor cell apoptosis and percentage of cells,cell cycle as well as the apparent diffusion coefficient (ADC) detected by magnetic resonance imaging (MRI) were observed. Results Among the 4 treated groups,the heaviest body mass and the de-tumor body mass of mice,and the lowest tumor index were presented in the BJD treated group (P<0.01 or P<0.05); ADC in the BJD treated group and the 5-FU treated group was lower than that in the BSP treated group and JPP treated group (P<0.05); the lowest VEGF level and tumor mass presented in the 5-FU group,and the highest presented in the JPP group (P<0.01 or P<0.05). Tumor inhibition rate and cell apoptosis rate in the 5-FU group was highest and in BJD group the secondary. As for cells of different cell cycles,comparisons between the 4 groups showed that S phase cell in 5-FU group<BJD group<BSP and JPP groups (P<0.01 or P<0.05); G0-G1 phase cell in BJD and BSP group<JPP group<5-FU group (P<0.01 or P<0.05). No significant difference of G2-M phase cell was shown among them. Conclusions The acting mechanism of BJD and its disassembled recipes on transplanted PCH may be inhibiting VEGF expression and accelerating tumor cell apoptosis. Change of MRI’s ADC value was keeping in step with changing of VEGF and cell apoptosis. Thereby,it could objectively reflect the metabolism of tumor cells,being a new means for assessing the effect of Chinese materia medica. |
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