| 张琦,余宏亮,于庆生,王汉明,帅剑锋,潘晋方.芪黄煎剂对缺血-再灌注大鼠肠黏膜上皮细胞Bcl-2、Bax及Caspase-3、9 mRNA表达的影响[J].,2011,31(2):223-227 |
| 芪黄煎剂对缺血-再灌注大鼠肠黏膜上皮细胞Bcl-2、Bax及Caspase-3、9 mRNA表达的影响 |
| Effect of Qihuang Decoction on mRNA Expressions of Bcl-2,Bax,and Caspase-3,9 in Intestinal Mucosa Epithelium of Ischemia/Reperfusion Injured Rats |
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| DOI： |
| 中文关键词: 芪黄煎剂 缺血/再灌注 肠黏膜 Bcl-2 Bax Caspase-3、9 mRNA |
| 英文关键词:Qihuang Decoction ischemia/reperfusion intestinal mucosa Bcl-2 Bax Caspase-3 mRNA Caspase-9 mRNA |
| 基金项目:安徽省高校自然科学基金重点项目(No.KJ2008A44ZC) |
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| 中文摘要: |
| 目的观察中药芪黄煎剂对缺血-再灌注(ischemia-reperfusion,I/R)损伤大鼠肠黏膜上皮细胞凋亡基因Bcl-2、Bax mRNA表达和信号转导分子Caspase-3、9 mRNA表达的影响。方法 40只Wistar大鼠随机分为假手术组、I/R损伤组、谷氨酰胺(Gln)组和芪黄煎剂组,每组10只。假手术组管饲生理盐水3天后,仅腹壁切开而不行I/R手术;I/R损伤组与假手术组同样方法干预后,行I/R损伤手术;Gln组和QHD组分别管饲Gln和芪黄煎剂3天后,行I/R损伤手术。RT-PCR方法检测肠上皮细胞凋亡基因Bcl-2、Bax mRNA表达和凋亡信号转导分子Caspase-3、9 mRNA表达。结果与假手术组比较,I/R损伤组大鼠肠黏膜细胞内Bcl-2、Bax、Caspase-3、9 mRNA均明显增多,差异有统计学意义(P<0.05)。与I/R损伤组比较,Gln组、芪黄煎剂组Bcl-2 mRNA表达增多,Bax、Caspase-3、9 mRNA表达减少,差异均有统计学意义(P<0.05)。与Gln组比较,芪黄煎剂组Bax mRNA表达更低(0.281±0.087vs0.350±0.053),Bcl-2/Bax值更高(1.648vs1.374),差异无统计学意义(P>0.05)。结论芪黄煎剂通过抑制细胞凋亡减轻I/R对大鼠肠黏膜上皮造成的损伤,其作用机制可能与增加Bcl-2 mRNA表达和降低Bax、Caspase-3、9 mRNA表达有关。 |
| 英文摘要: |
| Objective To observe the effect of Qihuang Decoction (QHD) on mRNA expression of apoptosis genes Bcl-2,Bax,and signal transduction molecules Caspase-3,9 in intestinal mucosa epithelium of ischemia/reperfusion (I/R) injured rats. Methods Forty Wistar rats were randomized equally into 4 groups,the control group,the model group,the glutamine group,and the QHD group. Rats in the latter two groups were gastric infused with glutamine and QHD respectively for 3 days,but saline was infused instead to rats in the control group and model group. After then,except those in the control group intervened only by sham operation,rats were made into I/R injured model by 45 min occlusion of superior mesenteric artery followed by 1 h reperfusion. Immediately after modeling,mRNA expressions of Bcl-2,Bax,Caspase-3,and Caspase-9 in intestinal mucosa epithelium of rats were detected by reverse transcription-polymerase chain reaction (RT-PCR). Results Compared with the control group,mRNA expressions of Bcl-2,Bax,Caspase-3 and Caspase-9 were higher in the other three groups (P<0.05). Compared with the model group,Bcl-2 mRNA expression was higher,while the expressions of the other three indices were lower in both the glutamine group and the QHD group (P<0.05); and comparisons between the glutamine group and the QHD group showed a more depressed Bax mRNA expression (0.281±0.087 vs 0.350±0.053) and higher Bcl-2/Bax ratio (1.648 vs 1.374) in the QHD group. Conclusions QHD can reduce the I/R injury in the intestinal mucosa epithelium by inhibiting the cell apoptosis. The mechanism may be correlated with increased Bcl-2 mRNA expressions and decreased mRNA expressions of Bax,Caspase-3 and Caspase-9. |
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