| 赵喜新,秦庆广,路玫,李建伟,李道明,金玉晶,高杨,曹大明.针灸对环磷酰胺所致骨髓抑制小鼠骨髓细胞周期调节蛋白Cyclin D1表达及细胞周期的动态影响[J].,2011,31(2):238-243 |
| 针灸对环磷酰胺所致骨髓抑制小鼠骨髓细胞周期调节蛋白Cyclin D1表达及细胞周期的动态影响 |
| Study on Dynamic Effect of Acupuncture on Marrow Cell Cycle Regulatory Protein Cyclin D1 Expression and Cell Cycle in Mice with Cyclophosphamide Induced Myelosuppression |
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| DOI： |
| 中文关键词: 针灸 机理研究 环磷酰胺 骨髓抑制 Cyclin D1 细胞周期 |
| 英文关键词:acupuncture and moxibustion mechanism study cyclophosphamide myelosuppression cyclin D1 cell cycle |
| 基金项目:国家自然基金重大计划(No.90709035) |
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| 中文摘要: |
| 目的探讨针灸抗化疗毒副反应,改善骨髓抑制、提高外周血白细胞数量的分子生物学机制。方法 224只清洁级、雄性昆明种小鼠,随机分为正常组、模型组、针刺组、艾灸组。采用公认的环磷酰胺(cyclophosphamide,CTX)骨髓抑制模型。针刺组、艾灸组分别采用针刺、艾灸治疗,每天1次,连续7天。正常组、模型组每天陪同针刺组、艾灸组抓取、固定,不做任何治疗。各组分别于治疗后第2～7天采用免疫组化法观察骨髓细胞周期调节蛋白Cyclin D1表达、流式细胞仪检测各细胞周期的骨髓细胞百分率。结果治疗后第5天,针刺组和艾灸组CTX小鼠的白细胞计数较模型组提前1天回升并超过基础水平;针刺组、艾灸组可以上调CTX小鼠骨髓细胞周期调节蛋白Cyclin D1的表达,并于治疗后第4天升至最高,与模型组比较差异有统计学意义(P<0.01);针刺组、艾灸组Gl期骨髓细胞百分率始终低于模型组,且在治疗后第2～4天比较差异均有统计学意义(P<0.01,P<0.05),S期、G2-M期骨髓细胞百分率始终高于模型组。结论烷化剂CTX损伤骨髓细胞的同时,干扰了正常的细胞周期规律,使DNA含量减少,骨髓抑制,外周血白细胞降低。针刺与艾灸可以促进骨髓细胞周期调节蛋白Cyclin D1表达,加速细胞从G1期向S期的转化,增强细胞DNA的合成,同时利用细胞G2期阻滞的时机,尽快修复受损细胞DNA,加速细胞有丝分裂,进入增殖状态,提高机体细胞抗损伤和修复的能力,保护骨髓细胞,改善骨髓抑制状态。 |
| 英文摘要: |
| Objective To explore the molecular biological mechanism of acupuncture and moxibustion (A&M) in reducing chemotherapy-related toxicity,relieving myelosuppression and increasing peripheral white blood cells (WBC). Methods Two hundred and twenty-four male Kunming mice of clean grade were randomized equally into 4 groups,the blank control group (A),the model group (B),the acupuncture group (C),and the moxibustion group (D). Except those in Group A,mice were duplicated into myelosuppression model with cyclophosphamide (CTX) using the accepted method. After being modeled,mice in Group C and D were treated with acupuncture and moxibustion respectively,once a day for 7 successive days,while those in Group A and B were dealt with the same actions (seizing and fixing) every day but no therapy was given. From day 2 to day 7 of the treatment,8 mice were taken from every group per day and killed in batches. Their peripheral WBC was counted and bone marrow for detecting Cyclin D1 expression and percentages of bone marrow cells in different cycle stages using immunohistochemistry and flow cytometer respectively. Results WBC count restored to exceed the baseline in group C and D at day 5 of the treatment,being one day earlier than that in group B. Cyclin D1 expression in the bone marrow raised in Group C and D,and reached the peak at day 4,showing significant difference as compared with that in Group B (P<0.01). The phase G1 marrow cell percentages in Group C and D was lower than that in Group B at all days of detection,showing statistical significance at day 2-4 (P<0.05 or P<0.01); while the percentages of phase S and G2-M cells in the two treated groups was higher than that in group B all the times. Conclusions While CTX damaged marrow cells,it intervened the cell cycle regularity and reduced the DNA content to cause myelosuppression and leucocytopenia. A&M therapy could improve the Cyclin D1 expression,speed up the cell transition from phase G1 to phase S and increase the synthesis of DNA. At the mean time,taking advantage of the block at phase G2,it can repair the injured DNA,speed up cell mitosis for turning into multiplication,improve the anti-injury and repairing ability of cells to protect bone marrow cells,and relieve the myelosuppression. |
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