| 张红英,徐长青,孙婧,吕玉宝,李璐璐,杜文静,董竞成,蔡萃.淫羊藿苷对哮喘小鼠肺内嗜酸性粒细胞凋亡和Bcl-2、Bax基因蛋白表达的影响[J].,2011,31(9):1248-1253 |
| 淫羊藿苷对哮喘小鼠肺内嗜酸性粒细胞凋亡和Bcl-2、Bax基因蛋白表达的影响 |
| Effects of Icariin on Bcl-2 and Bax Protein Expressions and Eosinophils Apoptosis in Bronchial Asthmatic Mice |
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| DOI： |
| 中文关键词: 支气管哮喘 淫羊藿苷 嗜酸性粒细胞 细胞凋亡 Bcl-2和Bax |
| 英文关键词:bronchial asthma icariin eosinophil apoptosis Bcl-2 and Bax |
| 基金项目:国家重点基础研究发展计划973计划项目(No.2009CB523001);上海市卫生局中医药科研基金项目(No.2008L018A);2009年度上海市优秀学科带头人计划(A类)项目(No.09XD1400700);新教师基金课题(No.200802461053) |
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| 中文摘要: |
| 目的观察淫羊藿苷对支气管哮喘小鼠肺组织嗜酸性粒细胞(EOS)凋亡及相关基因蛋白Bcl-2、Bax表达的影响。方法雌性Balb/c小鼠48只随机分为6组,即正常对照组、哮喘模型组、地塞米松组、淫羊藿苷低剂量组、淫羊藿苷中剂量组、淫羊藿苷高剂量组,每组8只。除正常对照组外以卵蛋白致敏并吸入激发法制备小鼠支气管哮喘模型,各治疗组均从第1次哮喘激发开始(造模第3周)至处死前隔天腹腔注射给药并激发,激发并给药6周后处死小鼠。应用TUNEL法检测肺组织EOS凋亡,免疫组织化学法检测肺组织Bcl-2、Bax蛋白的表达。结果与哮喘模型组比较,各用药组小鼠肺内EOS凋亡指数显著升高(P<0.05);肺组织及气道壁Bcl-2蛋白阳性面积显著降低(P<0.05);Bax蛋白阳性面积显著增加(P<0.05)。结论淫羊藿苷可调节抑凋亡基因蛋白Bcl-2和促凋亡基因蛋白Bax的表达,促进肺内EOS凋亡,减少EOS浸润。 |
| 英文摘要: |
| Objective To study the effects of icarrin on Bcl-2 and Bax protein expressions and eosinophils apoptosis in bronchial asthmatic mice.Methods 48 female Balb/c mice were randomly divided into 6 groups,i.e.,the normal control group,the model group,the Dexamethasone group,the low dose icariin group,the middle dose icariin group,and the high dose icariin group,8 mice in each group.Bronchial asthma in mice were induced by intraperitioneal sensitization and challenged with nebulized ovalbumin(OVA).The mice of each treatment group were administrated with different doses of icariin by peritoneal injection from the first asthma sensitization(the 3rd week after the modeling) to the day before killing once every other day,while mice in the normal control group were administrated with physiological saline.The mice were killed after 6 weeks of treatment.The apoptosis of eosinophils and the Bcl-2 and Bax protein expressions of the lung tissues were detected by TUNEL and immunohistochemical assay respectively.Results As compared with the model group,the apoptosis ratio of eosinophils were higher in the rest four treatment groups(P<0.05).The Bcl-2 protein positive areas in the lung tissues and the airway wall were significantly lowered(P<0.05).The Bax protein positive area significantly increased(P<0.05).Conclusion In bronchial asthmatic mice,icariin could enhance the apoptosis of eosinophils and lessen their infiltration by decreasing the expression of Bcl-2 protein and increasing the expression of Bax protein in lung. |
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