| 张海男,张春虎,王杨,黄熙,胡随瑜,邓颖,程田力.柴胡疏肝散及其拆方对抑郁模型大鼠行为及海马、杏仁核、额叶BDNF及其受体TrkB的影响[J].,2011,31(10):1373-1378 |
| 柴胡疏肝散及其拆方对抑郁模型大鼠行为及海马、杏仁核、额叶BDNF及其受体TrkB的影响 |
| Effects of Chaihu Shugan Powder on the Behavior and Expressions of BDNF and TrkB in the Hippocampus,Amygdala,and the Frontal Lobe in Rat Model of Depression |
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| DOI： |
| 中文关键词: 柴胡疏肝散 抑郁模型 脑源性神经营养因子 酪氨酸激酶受体B |
| 英文关键词:Chaihu Shugan Powder depression model brain derived neurophic factor tyrosine kinase receptors B |
| 基金项目:国家自然科学基金青年基金资助课题(No.30801506) |
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| 中文摘要: |
| 目的探讨柴胡疏肝散对抑郁模型大鼠行为学及海马、杏仁核、额叶皮质脑源性神经营养因子(brain derived neurophic factor,BDNF)及其受体酪氨酸激酶受体B(TrkB)表达的影响。方法 60只成年SD大鼠随机分为正常对照组(正常组)、模型对照组(模型组)、柴胡疏肝散组(中药组)、拆方干预Ⅰ组(拆方Ⅰ组)、拆方干预Ⅱ组(拆方Ⅱ组)及氟西汀对照组(西药组),每组10只;采用慢性轻度不可预见性应激加孤养复制抑郁模型;所有大鼠在造模第15天开始灌胃,分别灌服等体积生理盐水(正常组、模型组)及相应药液(中药组:5.9g/kg,拆方Ⅰ组:3.3g/kg,拆方Ⅱ组:2.6g/kg,西药组1.8mg/kg),连续2周。于实验第0、7、14、21、28天观察各组大鼠体重、糖水消耗及敞箱实验得分;免疫组化及实时荧光定量PCR检测海马、额叶皮质、杏仁核BDNF及TrkB mRNA表达。结果与正常组比较,模型组大鼠体重增加缓慢,敞箱实验得分降低,理毛次数及糖水消耗均减少,中央格停留时间延长;海马、额叶皮质、杏仁核区BDNF及TrkB mRNA表达降低,差异有统计学意义(P<0.05,P<0.01);与模型组比较,柴胡疏肝散组及各拆方组和西药组大鼠行为学指标显著改善,海马、额叶皮质、杏仁核区BDNF及TrkB表达增强,差异有统计学意义(P<0.05,P<0.01)。结论柴胡疏肝散可明显改善抑郁模型大鼠的抑郁状态,其机制可能与增加海马、额叶、杏仁核区BDNF及其受体TrkB mRNA表达有关。 |
| 英文摘要: |
| Objective To investigate the effects of Chaihu Shugan Powder(CHSGP) on the behavior and the expressions of brain-derived neurophic factor(BDNF) and its receptor tyrosine kinase receptors B(TrkB) in the hippocampus,amygdala,and the frontal lobe of depression model rats.Methods Sixty adult Sprague-Dawley rats were randomly divided into 6 groups,i.e.,the normal control group(NC),the model control group(MC),the CHSGP group,the disassembly 1 group(CⅠ),the disassembly 2 group(CⅡ),and the Fluoxetine control group(FC),10 in each group.Except those in the NC,the rest rats were singly housed and exposed on an unpredicted sequence of mild stressor.From the fifteenth day,all rats were administered with equal volume of normal saline(to the NC group and the MC group) and of corresponding medicinal liquid(5.9 g/kg to the CHSGP group,3.3 g/kg to the CⅠ group,2.6 g/kg to the CⅡ group,and 1.8 mg/kg to the FC group) by gastrogavage for 2 successive weeks.The rats’ body weight,sucrose consumption volume in the sucrose preference test,and times of grooming in the open field test were detected on the 0,7th,14th,21st,28th day,respectively.The mRNA expressions of BDNF and TrkB in the hippocampus,amygdala,and the frontal lobe were detected by immunohistochemical assay and Real-time fluorescent quantitation PCR.Results Compared with the NC group,the rats’ body weight was put up slowly in the MC group.The scores in the open field test decreased.The times of grooming and sucrose consumption volume were both reduced.The time of staying in central square was postponed.The mRNA expressions of BDNF and TrkB in the hippocampus,amygdala,and the frontal lobe decreased with statistical significance(P<0.05,P<0.01).Compared with the MC group,the behavior indices of rats in the CHSGP,CⅠ,CⅡ,and FC groups were significantly improved.The mRNA expressions of BDNF and TrkB in the hippocampus,amygdala,and the frontal lobe were obviously enhanced with statistical significance(P<0.05,P<0.01).Conclusions CHSGP could obviously improve the depressive state of the model rats.Its mechanism might be correlated with increasing the mRNA expressions of BDNF and TrkB in the hippocampus,amygdala,and the frontal lobe. |
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