| 陈志炉,戴其舟,王勇,任莉,蒋慧芳,周郁鸿.紫草素对白血病细胞HL-60增殖及凋亡作用的影响[J].,2012,32(2):239-243 |
| 紫草素对白血病细胞HL-60增殖及凋亡作用的影响 |
| Effects of Shikonin on the Proliferation and Apoptosis of HL-60 Cells |
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| DOI： |
| 中文关键词: 紫草素 HL-60细胞 细胞凋亡 bcl-2 |
| 英文关键词:shikonin HL-60 cell apoptosis bcl-2 |
| 基金项目:浙江省中医药科技计划项目(No.2008CA025);浙江省医药卫生科技项目(No.2008B032) |
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| 中文摘要: |
| 目的研究紫草素诱导人早幼粒白血病HL-60细胞凋亡的作用机制。方法 MTT比色法检测紫草素对HL-60细胞增殖的影响;Annexin V/PI分析凋亡率;半定量逆转录-聚合酶链反应(RT-PCR)检测bcl-2基因水平,分析白血病HL-60细胞凋亡作用与bcl-2表达水平关系。结果紫草素在1～8μg/mL浓度范围内能抑制HL-60细胞的增殖,具有时间和浓度的依赖性。2μg/mL紫草素能够诱导HL-60细胞凋亡,凋亡呈时间依赖性。在2μg/mL紫草素作用下,HL-60细胞bcl-2表达明显下调。结论紫草素能够诱导HL-60细胞凋亡,其作用机制与下调bcl-2表达有关。 |
| 英文摘要: |
| Objective To explore the mechanism of shikonin for inducing the apoptosis of human promyelocytic leukemia cell HL-60.Methods The effects of shikonin on the HL-60 cell proliferation were detected using MTT.The apoptosis rate was analyzed by Annexin-Ⅴ/PI double staining.The expression level of the bcl-2 gene was detected using semi-quantitative reverse transcriptase PCR(RT-PCR),thus analyzing the correlation between the bcl-2 expression level and the apoptosis of HL-60.Results Shikonin could inhibit the proliferation of HL-60 cells with the concentration range of 1～8 μg/mL in a time-and concentration-dependent manner.Two μg/mL shikonin could induce the apoptosis of HL-60 cells in a time-dependent manner.The expression level of bcl-2 was obviously down-regulated at 2 μg/mL shikonin.Conclusions Shikonin could induce the apoptosis of HL-60 cells.Its mechanism was correlated with down-regulation of the expression level of bcl-2. |
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