| 郝丽,潘梦舒,郑云,王瑞峰.冬虫夏草及雷公藤多甙对糖尿病肾病大鼠足细胞影响的实验研究[J].,2012,32(2):261-265 |
| 冬虫夏草及雷公藤多甙对糖尿病肾病大鼠足细胞影响的实验研究 |
| Effects of Cordyceps Sinensis and Tripterygium wilfordii Polyglycosidium on the Podocytes in Rats with Diabetic Nephropathy |
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| DOI： |
| 中文关键词: 糖尿病肾病 冬虫夏草 雷公藤多甙 大鼠足细胞 |
| 英文关键词:diabetic nephropathy Cordyceps Sinensis Tripterygium Wilfordii Polyglycosidium rat podocyte |
| 基金项目:安徽省自然科学基金资助项目(No.070413066) |
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| 中文摘要: |
| 目的探讨冬虫夏草及雷公藤多甙对糖尿病肾病(DN)大鼠足细胞的保护作用。方法制备DN大鼠模型,将动物随机分为正常对照组(A组)、DN模型组(B组)、冬虫夏草治疗组(C组)及雷公藤多甙治疗组(D组)、两药联合治疗组(E组)。12周后观察24h尿蛋白排泄量(24hurinary protein count,24hpro)、血糖(Glu)、血尿素氮(BUN)、血肌酐(SCr)、外周血白细胞(WBC)、谷草转氨酶(AST)、谷丙转氨酶(ALT)以及肾重(KW)/体重(BW)等值的变化;HE染色检测肾组织病理变化;透射电镜测量足细胞超微结构变化;免疫荧光方法检测肾皮质nephrin和podocin蛋白表达。结果与A组比较,B组SCr、BUN增高(P<0.05);Glu、KW/BW和24hpro明显增高(P<0.01),肾小球、小管间质及足细胞病变,肾脏皮质nephrin和podocin蛋白表达减少。与B组比较,C、D、E组KW/BW和24hpro降低(P<0.01),肾小球、小管间质及足细胞病变明显减轻;肾脏皮质nephrin和podocin蛋白表达增高。与C组比较,D、E组KW/BW和24hpro降低,肾小球、小管间质及足细胞病变减轻,肾脏皮质nephrin和podocin蛋白表达增高,差异均有统计学意义以E组最为明显(P<0.01)。结论冬虫夏草及雷公藤多甙具有减轻DN蛋白尿,保护及修复DN大鼠足细胞病变的作用。其机制可能与其上调nephrin和podocin蛋白的表达有关。冬虫夏草与雷公藤多甙联合治疗可增加疗效并减轻雷公藤多甙的不良反应。 |
| 英文摘要: |
| Objective To investigate the effects of Cordyceps Sinensis(CS) and Tripterygium wilfordii Polyglycosidium(TWP) on the podocytes in rats with diabetes nephropathy(DN).Methods Rat models of DN were established and rats were randomly divided into the normal control group(Group A),the DN group(Group B),the CS group(Group C),the TWP group(Group D),and the CS combined TWP group(Group E).The changes of 24-h urinary protein count(24-h pro),blood glucose(Glu),blood urea nitrogen(BUN),serum creatinine(SCr),white blood cell(WBC),aspartate aminotransferase(AST),alanine aminotransferase(ALT),and kidney weight/body weight(KW/BW) were determined 12 weeks later.The renal pathological changes were evaluated by HE staining.The microstructural changes of podocytes were observed by transmission electron microscope.The expressions of nephrin and podocin were detected by immunofluorescence staining.Results Compared with Group A,the levels of SCr and BUN were higher(P<0.05),Glu,KW/BW,and 24 h pro were obviously higher in Group B(P<0.01).There were less glomerular disorder,chronic tubulointerstitial damage and glomerular podocyte lesion in Group B.The expressions of the massive cortical nephrin and podocin protein were obviously less in Group B.Compared with Group B,the KW/BW and 24 h pro in Group C,D,and E were lower(P<0.01).The pathomorphological improvement was exhibited in the glomerulus,tubules,and podocytes.The protein expressions of nephrin and podocin were higher in the renal cortex.Compared with Group C,the KW/BW and 24-h pro decreased in Group D and E,the expressions of nephrin and podocin were enhanced,and the lesions of the glomerulus,tubules,and podocytes were alleviated,showing statistical difference(P<0.01).The most significant changes happened in Group E.Conclusions CS and TWP could alleviate the DN proteinuria,protect and repair podocytes of DN rats.Its mechanisms might be correlated with up-regulating the expressions of nephrin and podocin.The combined use of CS and TWP could increase the efficacy and attenuate adverse reactions of TWP. |
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