| 王瑞,王磊,张卫星,秦俊昌,张杰.葛根素对钴60-γ射线致小鼠睾丸支持细胞急性损伤的保护作用[J].,2012,32(3):385-389 |
| 葛根素对钴60-γ射线致小鼠睾丸支持细胞急性损伤的保护作用 |
| Protective Effects of Puerarin on 60Co-γ-induced Acute Injury of sertoli Cells of Testis in Mice |
| |
| DOI： |
| 中文关键词: 葛根素 钴60-γ 睾丸支持细胞 |
| 英文关键词:puerarin 60Co-γ testicular sertoli cell |
| 基金项目:河南省科技攻关项目(No.102102310067) |
|
| Hits: 1369 |
| Download times: 5 |
| 中文摘要: |
| 目的探讨中药单体葛根素对钴60(60Co)-γ射线致小鼠睾丸支持细胞急性损伤的保护作用及机制。方法 40只健康雄性Wistar小鼠随机分为正常组,模型组及低、高剂量组,每组10只。模型组及低、高剂量组给予60Co-γ8.0Gy下腹部均匀照射517s。造模24h后,低、高剂量组分别给予葛根素230、450mg/(kg·d)灌胃,正常组和模型组给予等量生理盐水灌胃,均每天1次,连续2周。观察小鼠睾丸组织形态学变化;采用酶联免疫吸附法测量血清卵泡刺激素(follicle-stimulating hormone,FSH)、促黄体生成素(luteinizing hormone,LH)及抑制素β(Inhibinβ)水平;应用实时定量PCR法测定睾丸组织中Inhibinβ mRNA相对表达量。结果模型组小鼠睾丸支持细胞和生精细胞发生不同程度的损伤,低、高剂量组小鼠有不同程度的改善,以高剂量组改善最为明显。与正常组比较,模型组血清FSH水平显著提高,血清Inhibinβ水平及睾丸组织Inhibinβ mRNA表达水平显著降低,差异均有统计学意义(P<0.05)。与模型组比较,低、高剂量组小鼠血清FSH、Inhibinβ水平及睾丸组织Inhibinβ mRNA表达显著改善(P<0.05),以高剂量组改善最明显(P<0.01,P<0.05)。结论中药单体葛根素对60Co-γ射线致小鼠睾丸支持细胞急性损伤有一定的保护作用。 |
| 英文摘要: |
| Objective To study the protective effects of puerarin on 60Co-γ-induced acute injury of sertoli cells of testis in mice and the mechanisms for its radiation protection.Methods Forty male Wistar mice were randomly divided into four groups,i.e.,the normal group,the model group,the low dose puerarin group,and the high dose puerarin group,respectively,10 in each.The lower abdomen and scrotal area of mice were irradiated with a single dose of 8 Gy 60Co-γ ray for 517 s in the model and two puerarin groups.Twenty-four h after modeling,different concentrations of puerarin(at the daily dose of 230 and 450 mg/kg respectively) were given to mice in the low and high dose groups by gastrogavage,once daily for two successive weeks.Equal volume of physiological saline was given to mice in the normal group and the model group,once daily for two successive weeks.The morphology of the testicular tissues was observed.The levels of serum follicle-stimulating hormone(FSH) ,luteinizing hormone(LH) ,and inhibin β were determined by ELISA.The relative expressions of Inhibinβ mRNA in the testicular tissues were determined by Real-time quantitative PCR.Results Sertoli cells and spermatogenic cells were injured at different degrees in the model group.They were improved in the low and high dose puerarin groups,more obviously in the high dose puerarin group.Compared with the normal group,the level of serum FSH in the model group was significantly improved,while the serum level of Inhibin β and the expression of Inhibin β mRNA obviously decreased,showing statistical difference(P<0.05) .Compared with the model group,the levels of serum FSH,Inhibin β,and the expression of Inhibin β mRNA were significantly improved in the low and high dose puerarin groups(P<0.05) ,more obviously in the high dose puerarin group(P<0.01,P<0.05) .Conclusion Chinese medicine monomer of puerarin had certain protective effects on 60Co-γ-induced acute injury of Stetoli cells in mice. |
| View Full Text View/Add Comment Download reader |
|
|
|
