| 吴生兵,何光远,周美启,曹健,高纺,杨影,曹丽丽.电针对脑心综合征大鼠脑组织细胞凋亡及Caspase-3表达的影响[J].,2012,32(5):639-642 |
| 电针对脑心综合征大鼠脑组织细胞凋亡及Caspase-3表达的影响 |
| Effects of Electroacupuncture on the Apoptosis of Brain Tissue Cells and the Expression of Caspase-3 in the Rats with Cerebral-Cardiac Syndrome |
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| DOI： |
| 中文关键词: 电针 脑心综合征 细胞凋亡 半胱氨酸天冬氨酸蛋白酶3 |
| 英文关键词:electroacupuncture cerebral-cardiac syndrome cell apoptosis cysteine containing aspartate specific protease-3 |
| 基金项目:安徽省自然科学基金项目(No.11040606M188);安徽省人才开发专项资金项目(No.2008Z036);安徽中医学院自然科学研究项目(No.2011zr009B) |
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| 中文摘要: |
| 目的观察电针对脑心综合征(cerebral-cardiac syndrome,CCS)大鼠血肿周围脑组织细胞凋亡及Caspase-3表达的影响。方法从70只健康SD大鼠中随机选择10只作为假手术组,其余60只用于CCS模型复制。选取造模成功的大鼠30只,分为模型组、电针组和电针非经非穴组,每组10只。采用胶原酶加肝素联合注射大鼠尾状核方法造模,假手术组向大鼠尾状核注入等量0.9%氯化钠注射液。于造模第1天开始电针,电针组选取水沟、风府、内关和心俞穴,电针非经非穴组选取臀部非经非穴点4处,每次20min,每天1次,连续3天。假手术组和模型组仅每天抓空1次。分别采用TUNEL法和免疫组化法检测血肿周围脑组织细胞凋亡及Caspase-3表达。结果假手术组偶可见TUNEL细胞,Caspase-3少量表达;模型组血肿周围组织出现较多的TUNEL阳性细胞,Caspase-3大量表达;电针组TUNEL阳性细胞和Caspase-3表达均较模型组和电针非经非穴组明显减少(P<0.01)。结论电针可以抑制CCS大鼠脑组织细胞凋亡,其机制可能与下调血肿周围脑组织Caspase-3表达有关。 |
| 英文摘要: |
| Objective To observe the effects of electroacupuncture(EA)on the apoptosis of brain tissue cells and the expression of Caspase-3 in the rats with cerebral-cardiac syndrome(CCS).Methods A total of 70 healthy SD rats were selected.Ten was randomly recruited as the sham-operation group,and the rest were used for CCS model preparation.Thirty successfully modeled rats were divided into the model group,the EA group,and the non-EA group,10 in each group.The model was prepared using injecting collagenase+heparin into the caudate nucleus.Equal volume of 0.9% sodium chloride injection was injected to rats’ caudate nucleus in the sham-operation group.EA was started on the 1st day of modeling.Shuigou(GV26),Fengfu(GV16),Neiguan(PC6),and Xinshu(BL15)were needled in the EA group.Four points in the hips were needled in the non-EA group.The EA needling lasted for 20 min each time,once daily,for 3 successive times.No EA was administered to the sham-operation group or the model group.The apoptosis of brain tissue around the hematoma and the expression of Caspase-3 were detected using TUNEL and immunochemical assay.Results TUNEL cells could be occasionally seen with fewer Caspase-3 expression in the sham-operation group.More TUNEL positive cells appeared in the tissue around the hematoma of the model group with a large amount of Caspase-3 expression.The TUNEL positive cells and Caspase-3 expression were obviously less in the EA group than in the model group and the non-EA group(P<0.01).Conclusions EA could inhibit the apoptosis of brain tissue cells in CCS rats.Its mechanisms might be associated with down-regulating the Caspase-3 expression of the brain tissue around the hematoma. |
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