| 赵桂峰,范英昌,姜希娟.丹酚酸B对内皮祖细胞与骨髓间充质干细胞联合心脏移植后增殖状况的影响[J].,2012,32(5):671-675 |
| 丹酚酸B对内皮祖细胞与骨髓间充质干细胞联合心脏移植后增殖状况的影响 |
| Effects of the Proliferation State of the Endothelial Progenitor Cells Preconditional with Salvianolic Acid B and Bone Marrow Mesenchymal Stem Cells Transplanted in Acute Myocardial Infarction Rats |
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| DOI： |
| 中文关键词: 丹酚酸B 内皮祖细胞 骨髓间充质干细胞 细胞移植 |
| 英文关键词:salvianolic acid B endothelial progenitor cell bone marrow mesenchymal stem cell cellular transplantation |
| 基金项目:国家自然科学基金资助项目(No.30572443) |
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| 中文摘要: |
| 目的通过丹酚酸B预处理的内皮祖细胞(endothelial progenitor cells,EPCs)与骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)混合后对心肌梗死大鼠进行心脏移植,观察移植细胞的增殖情况。方法密度梯度离心法和贴壁筛选法培养、纯化EPCs与BMSCs;免疫细胞化学法(CD34、CD133、CD44)分别鉴定2种细胞。采用左冠状动脉结扎法制作大鼠急性心肌梗死模型。丹酚酸B最佳药物浓度(8μg/mL)预处理EPCs,并以不同比例与BMSCs混合(EPCs/BMSCs分别为1∶1、2∶1、4∶1和8∶1),手术移植至心肌梗死区。HE和N-BT染色法检测心肌梗死面积;免疫组化法检测Ki-67的表达情况。结果与模型组(19.60%±3.23%)比较,各细胞移植组心肌梗死面积均明显下降(P<0.05),其中4∶1组(11.37%±2.18%)和8∶1组(9.23%±2.35%)效果最明显(P<0.05)。与模型组[(5.17±2.31)个/高倍视野]比较,各细胞移植组Ki-67阳性细胞数均显著增多(P<0.05),其中8∶1组阳性细胞数[(15.00±3.16)个/高倍视野]明显高于其他细胞移植组(P<0.05)。结论丹酚酸B预处理的EPCs联合BMSCs进行心脏移植,能够减少大鼠心肌梗死面积,提高BMSCs在梗死周边和缺血局部的增殖。并且随着EPCs移植比例的增加,梗死面积逐渐减少,增殖表达逐渐增强。 |
| 英文摘要: |
| Objective To observe the proliferation state of transplanted cells in acute myocardial infarction(AMI)rats,and the endothelial progenitor cells(EPCs)preconditioned by salvianolic acid B in different ratios with the bone mesenchymal stem cells(BMSCs).Methods The cultivation and purification of EPCs were performed by density-gradient centrifugation and plastic adherence method.Two types of cells were identified by immunocytochemical method(CD34,CD133,and CD44).The rat model of AMI was prepared by ligation of left anterior descending artery.The EPCs were pre-treated with the optimal concentration of salvianolic acid B(8 μg/mL).They were mixed with BMSCs in different proportions(EPCs/BMSCs in the ratio of 1:1,2:1,4:1,and 8:1,respectively).BMSCs and EPCs were injected into the myocardial infarction area.The infarcted area was determined by the N-BT staining and hematoxylin-eosin staining.The expression of Ki-67 was detected by immunohistochemical assay.Results Compared with the model group(19.60%±3.23%),the myocardial infarction area of each implanted group obviously decreased(P<0.05).Of them,the decrease was most obvious in the 4:1 group(11.37%±2.18%)and the 8:1 group(9.23%±2.35%,P<0.05).Compared with the model group(cell/high magnification,5.17±2.31),the Ki-67 positive cell number of each implanted groups significantly increased(P<0.05).Of them,the Ki-67 positive cell number was obviously higher in the 8:1 group(15.00±3.16,P<0.05).Conclusions EPCs pretreated by salvianolic acid B combined with BMSCs could reduce the myocardial infacted area,improve the proliferation of BMSCs in the peripheral infarction and local ischemia.Besides,along with the increase of the implant proportion of EPCs,the infarct area was gradually reduced,and the proliferative expression was gradually enhanced. |
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