| 王兵,曹书华,王勇强,胡才理,徐新女.加味凉膈散含药血清对脂多糖刺激小鼠血小板Toll样受体4表达及细胞因子释放的影响[J].,2012,32(5):681-684 |
| 加味凉膈散含药血清对脂多糖刺激小鼠血小板Toll样受体4表达及细胞因子释放的影响 |
| Effects of Modified Liangge Powder Contained Serum on LPS Stimulated TLR4 Expression and Release of Cytokines in Mouse Platelets |
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| DOI： |
| 中文关键词: 血小板 Toll样受体4 脂多糖 加味凉膈散 |
| 英文关键词:platelet Toll like receptor-4 lipopolysaccharide Modified Liangge Powder |
| 基金项目:天津市卫生局科技基金资助项目(No.06KZ72) |
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| 中文摘要: |
| 目的观察加味凉膈散对小鼠血小板Toll样受体4(toll like receptor 4,TLR4)表达及对血小板源细胞因子IL-8、β血小板球蛋白(β-TG)、可溶性CD40配体(sCD40L)释放量的影响。方法比较脂多糖(LPS)刺激TLR4单克隆抗体封闭和不封闭的血小板对细胞因子释放的影响。应用加味凉膈散低(0.94g/mL)、中(1.89g/mL)、高(2.84g/mL)剂量含药血清孵育LPS刺激的血小板,观察血小板TLR4表达及血小板源细胞因子释放量的变化。结果 LPS刺激后血小板TLR4阳性表达率明显降低(P<0.01),sCD40L和β-TG释放量显著升高(P<0.01);加入TLR4单克隆抗体后,sCD40L和β-TG释放量明显下降(P<0.05,P<0.01)。LPS刺激前后IL-8浓度比较,差异无统计学意义(P>0.05)。与LPS组比较,加味凉膈散中、高剂量含药血清组血小板TLR4阳性表达率明显升高(P<0.01);血小板sCD40L和β-TG的释放量明显下降(P<0.01,P<0.05),且随剂量增加抑制作用增强。结论 LPS通过血小板TLR4诱导血小板活化并释放细胞因子sCD40L、β-TG,而血小板IL-8的释放不依赖于血小板TLR4-LPS途径;加味凉膈散能抑制LPS刺激的血小板释放sCD40L、β-TG,并以剂量依赖方式抑制LPS与血小板TLR4的结合,减少血小板细胞因子的释放。 |
| 英文摘要: |
| Objective To observe the effects of Modified Liangge Powder(MLP)on the expressions of platelet toll like receptor 4(TLR4)and the release of platelet-derived cytokines interleukin 8(IL-8),β platelet globulin(β-TG),soluble CD40 ligand(sCD40L).Methods The modulating effects on the release of cytokines from mice platelets by TLR4 ligand through monoclonal antibody blocking TLR4 on platelet were compared.The stimulated platelet by LPS was incubated with low(0.94 g/mL),medium(1.89 g/mL),and high(2.84 g/mL)dose of MLP contained serum.The changes of the platelet TLR4 expression and platelet-derived cytokines were observed.Results The positive expression rate of platelet TLR4 obviously decreased(P<0.01)and the release of sCD40L and β-TG from platelets significantly increased(P<0.01)after stimulated by LPS.However,the release of sCD40L and β-TG from platelets obviously decreased by TLR4 monoclonal antibody(P<0.05,P<0.01).There was no statistical difference in IL-8 between before and after LPS stimulation(P>0.05).Platelet TLR4 positive expression rate was significantly higher after incubated by medium and high doses of MLP contained serum(P<0.01),and the releasing of sCD40L and β-TG was lower in the serum contained groups.The inhibitory effects were enhanced in a dose-dependent manner.Conclusions LPS induced platelet activation by TLR4 and released sCD40L and β-TG,while the release of platelet IL-8 was not dependent on platelet TLR4-LPS pathway.MLP could inhibit LPS-stimulated sCD40L and β-TG,inhibit the binding of platelet TLR4 and LPS in a dose-dependent manner,thus reducing the release of platelet cytokines. |
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