| 吕凌,贾连群,马巍,宋雪娇,崔永霞,王彩霞.脾气虚证大鼠回肠组织蛋白质差异性表达的实验研究[J].,2012,32(5):685-688 |
| 脾气虚证大鼠回肠组织蛋白质差异性表达的实验研究 |
| Experimental Study on the Ileum Tissue Proteins Differentially Expressed in Pi-qi Deficiency Syndrome Rats |
| |
| DOI： |
| 中文关键词: 脾气虚证 回肠 蛋白质组 |
| 英文关键词:Pi-qi deficiency syndrome ileum proteomics |
| 基金项目:国家自然科学基金资助项目(No.30973689) |
|
| Hits: 1500 |
| Download times: 6 |
| 中文摘要: |
| 目的采用蛋白质组学技术研究脾气虚证大鼠回肠组织蛋白的差异性表达。方法 37只大鼠随机分成3组,即正常对照组、脾气虚证模型组和利血平组。分别采用劳倦过度结合饮食不节和利血平注射液(1mg/mL)腹腔注射的方法建立脾气虚证动物模型,通过模糊数学方法鉴定后分离回肠组织,后采用双向电泳法分离差异表达的蛋白,凝胶银染后经选点、酶切和质谱分析,用MASCOT软件在蛋白质数据库中搜索肽质量指纹谱数据并确定蛋白质。结果脾气虚证模型组回肠组织蛋白质图谱中有3个蛋白点表达异常(P<0.05),利血平组回肠组织蛋白质图谱中有9个蛋白点表达异常(P<0.05)。结论脾气虚证的发生与白蛋白表达下调、胰蛋白酶和葡萄糖调节蛋白78表达上调有密切关系。 |
| 英文摘要: |
| Objective To identify the ileum tissue proteins differentially expressed in Pi-qi deficiency syndrome rats using proteomic approach.Methods Thirty-seven rats were randomly divided into 3 groups,i.e.,the normal control group(Group 1),the Pi-qi deficiency syndrome model group(Group 2),and the reserpine group(Group 3).The Pi-qi deficiency syndrome model was established using excessive exerting combined with irregular diet,and peritoneally injecting Reserpine Injection(1 mg/mL)respectively.The ileum tissues were separated after identified fuzzy method.The differentially expressed proteins were separated with two dimensional electrophoresis(2-DE),analyzed by Mass Spectrometry,and identified by MASCOT Software.Results Three proteins were differentially expressed in Group 2 and nine proteins were differentially expressed in Group 3(P<0.05).Conclusion Pi-qi deficiency syndrome was closely related with decreased expression of albumin as well as increased expressions of trypsin and glucose-regulated protein 78. |
| View Full Text View/Add Comment Download reader |
|
|
|
