| 李友建,夏俊,赵锐,孙淼,房兵,王惠.三氧化二砷对人乳腺癌SKBR-3细胞增殖及Notch1表达的影响[J].,2012,32(6):793-796 |
| 三氧化二砷对人乳腺癌SKBR-3细胞增殖及Notch1表达的影响 |
| Effects of Arsenic Trioxide on the Proliferation of Human Breast Cancer SKBR-3 Cell and the Expression of Notch1 |
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| DOI： |
| 中文关键词: 三氧化二砷 SKBR-3细胞 Notch1 细胞增殖 细胞迁移力 |
| 英文关键词:arsenic trioxide SKBR-3 cell Notch1 cell proliferation cell migration force |
| 基金项目:安徽省高等学校省级自然科学研究重点项目(No.KJ2012A196) |
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| 中文摘要: |
| 目的探讨三氧化二砷(arsenic trioxide,As2O3)对人乳腺癌SKBR-3细胞增殖和迁移力及Notch1表达的影响。方法以SKBR-3细胞为研究对象,以不同浓度As2O3培养24h和终浓度8μmol/L培养24、48、72h后,MTT比色法检测As2O3对SKBR-3细胞增殖的影响;Transwell检测As2O3对SKBR-3细胞迁移力的影响;RT-PCR检测Notch1mRNA表达;Western blot检测Notch1蛋白表达。结果 As2O3能显著抑制人乳腺癌SKBR-3细胞增殖,且呈现浓度、时间依赖关系(P<0.05);并能抑制SKBR-3细胞的迁移力(P<0.05);RT-PCR及Western blot结果显示As2O3作用SKBR-3细胞后,可使Notch1mRNA和蛋白的表达水平明显降低(P<0.05)。结论 As2O3能够抑制SKBR-3细胞Notch1的表达及抑制细胞增殖和迁移力,初步揭示As2O3可能是通过Notch1信号通路影响人乳腺癌细胞生物学行为,从而为临床砷剂治疗乳腺癌提供理论和实验依据。 |
| 英文摘要: |
| Objective To study the effects of arsenic trioxide(As2O3) on the proliferation and the migration force of human breast cancer SKBR-3 cell and the expression of Notch1.Methods SKBR-3 cells were cultured with different concentrations of As2O3 for 24 h and with the final concentration of 8 μmol/L for 24,48,and 72 h.The effects of As2O3 on the cell proliferation of SKBR-3 were detected by MTT assay.The effects of the migration force of SKBR-3 cells were detected by Transwell.The expression of Notch1 mRNA was detected using reverse transcription polymerase chain reaction(RT-PCR).The expression of Notch1 protein was detected using Western blot.Results As2O3 could significantly inhibit the proliferation of SKBR-3 cells in a concentration-and time-dependent manner(P<0.05).It also could inhibit the migration force of SKBR-3 cells(P<0.05).Results of RT-PCR and Western blot showed that Notch1 mRNA and protein levels obviously decreased(P<0.05).ConclusionAs2O3 could inhibit the expression of Notch1 and the cell proliferation and the migration force of SKBR-3 cells,which primarily revealed that As2O3 might affect the biological behavior of human breast cancer cells possibly through Notch1 signaling pathway,thus providing theoretical and experimental bases for treating breast cancer by arsenics. |
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