| 蔡青,黄淑芸,谭俊珍,周涛,李春深.丹参酮B钠盐对脑缺血再灌注损伤大鼠海马不同亚区NMDAR1蛋白表达的影响[J].,2012,32(8):1073-1076 |
| 丹参酮B钠盐对脑缺血再灌注损伤大鼠海马不同亚区NMDAR1蛋白表达的影响 |
| Effects of Sodium Tanshinone B on the Protein Expression of NMDAR1 in Rat Hippocampal Subfields Following Focal Ischemia/Reperfusion Injury |
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| DOI： |
| 中文关键词: 脑缺血再灌注 丹参酮B钠盐 海马 NMDAR CA1 CA3 |
| 英文关键词:cerebral ischemia/reperfusion injury sodium tanshinone B hippocampus N-methyl D-aspartate receptor CA1 CA3 |
| 基金项目:天津市高等学校科技发展基金计划项目(No.20070301) |
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| 中文摘要: |
| 目的观察大鼠脑缺血再灌注损伤后海马不同亚区神经元N-甲基-D-天冬氨酸受体(N-methyl D-aspartate receptor,NMDAR)蛋白表达变化规律及丹参酮B钠盐对其的影响,探讨丹参酮治疗脑缺血可能的作用机制。方法采用栓线法制备局灶性脑缺血再灌注动物模型,将Wistar大鼠随机分为假手术组、模型组、丹参酮B钠盐低、中、高剂量组。参照ZeaLongaEL的5级评分标准对其神经功能障碍进行评分;应用免疫组织化学方法检测缺血侧NMDAR1通道蛋白表达情况。结果神经功能缺损评分,丹参酮B钠盐中、高剂量组与模型组比较差异有统计学意义(P<0.01)。免疫组织化学结果显示:在CA1区,模型组、丹参酮B钠盐低剂量组、中剂量组NMDAR1的表达显著高于假手术组(P<0.01),丹参酮B钠盐高剂量组NMDAR1的表达显著低于模型组、丹参酮B钠盐低剂量组(P<0.01)和丹参酮B钠盐中剂量组(P<0.05)。在CA3区,模型组、丹参酮B钠盐低剂量组NMDAR1的表达显著高于假手术组、中剂量组和高剂量组(P<0.01),丹参酮B钠盐中、高剂量组NMDAR1的表达显著高于假手术组(P<0.05)。结论丹参酮B钠盐可促进脑缺血再灌注大鼠神经功能的恢复。丹参酮B钠盐通过降低兴奋性神经递质谷氨酸、减少NMDAR1通道蛋白表达而对抗脑缺血再灌注损伤。 |
| 英文摘要: |
| Objective To observe the changing laws of the protein expression of N-methyl D-aspartate receptor(NMDAR) in rat hippocampal subfields following focal ischemia/reperfusion injury,and to study the effects of sodium tanshinone B(STB) on it,thus exploring the possible mechanism of STB for treating cerebral ischemia.Methods The rat model of focal cerebral ischemia/reperfusion injury was established using middle cerebral artery occlusion(MCAO) by reversibly inserting a nylon thread.The Wistar rats were randomly divided into the sham-operation group,the I/R model group,and the low,middle,and high dose STB groups.The neural functional disturbance was scored referring to the 5-grade Zea Longa EL standard.The protein expression of NMDAR1 in the ischemic side was detected using immunohistochemical assay.Results There was statistical difference in the scores of the neural functional disturbance in the middle and high dose STB groups when compared with the model group(P<0.01).Results of the immunohistochemical assay showed the expression of NMDAR1 in CA1 region was obviously higher in the I/R model group,the low and middle dose STB groups than in the sham-operation group(P<0.01).The expression of NMDAR1 in CA1 region was obviously lower in the high dose STB group than in the I/R model group(P<0.01),the low(P<0.01) and middle dose STB groups(P<0.05).The expression of NMDAR1 in CA3 region was obviously higher in the low dose STB group and the I/R model group than in the sham-operation group,the middle and high dose STB groups(P<0.01).The expression of NMDAR1 in CA3 region was obviously higher in the high and middle dose STB groups than in the sham-operation group(P<0.05).Conclusions STB could promote the recovery of neural functions in cerebral ischemia/reperfusion injury rats.STB fought against cerebral ischemia/reperfusion injury by lowering excitable neurotransmitter glumatic acid and reducing the protein expression of NMDAR1. |
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