| 郏丹赟,张明晓,姚玉瑞,王均炉.人参皂苷Rb1对急性束缚性应激大鼠海马组织酪氨酸激酶B mRNA表达的影响[J].,2013,33(3):376-379 |
| 人参皂苷Rb1对急性束缚性应激大鼠海马组织酪氨酸激酶B mRNA表达的影响 |
| Effects of Ginsenoside Rb1 on the mRNA Expression of Tyrosine Kinase B in the Hippocampus of Acute Immobilization Stress Rats |
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| DOI： |
| 中文关键词: 急性束缚性应激 酪氨酸激酶B 人参皂苷Rb1 促肾上腺皮质激素和皮质酮 |
| 英文关键词:acute immobilization stress tyrosine kinase B ginsenoside Rb1 adrenocorticotropic hormone and corticosterone |
| 基金项目:浙江省自然科学基金资助项目（No.Y2101225）；温州市科技发展项目（No.Y20090254） |
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| 中文摘要: |
| 目的 观察急性束缚性应激对大鼠海马组织酪氨酸激酶B（tyrosine kinase B，TrkB） mRNA表达的影响。方法 将18只SD大鼠随机分成正常组、模型组和给药组，每组6只。模型组采用急性束缚2 h制备急性束缚性应激模型；给药组于造模前30 min给予人参皂苷Rb1（40 mg/kg）腹腔注射，余同模型组；正常组不作任何处理。采用ELISA法检测大鼠血浆促肾上腺皮质激素（adrenocorticotropic hormone，ACTH）和皮质酮（corticosterone，CORT）含量；实时荧光定量RT PCR检测大鼠海马组织TrkB mRNA表达水平。结果 造模前，各组血浆ACTH、CORT含量比较，差异无统计学意义（P>0.05）。造模后，模型组和给药组血浆ACTH、CORT含量均较本组造模前显著上升，差异有统计学意义（P<0.05）；且模型组明显高于正常组同期（P<0.05），给药组明显高于模型组同期（P<0.05）。与正常组比较，模型组海马组织TrkB mRNA表达水平下降（87.73±7.62 vs 50.65±5.19），差异有统计学意义（P<0.05）；给药组TrkB mRNA表达水平（78.91±18.07）较模型组升高，差异有统计学意义（P<0.05）。结论 人参皂苷Rb1预处理可增加大鼠血浆CORT和ACTH含量，并维持海马组织TrkB mRNA表达水平，从而缓解急性束缚性应激所导致的损害。 |
| 英文摘要: |
| Objective To observe the effects of acute immobilization stress on the mRNA expression of tyrosine kinase B （TrkB） in rats′ hippocampus. Methods Eighteen SD rats were randomly divided into three groups， i.e.， the normal control group， the model group， and the medication group， 6 in each group. The acute immobilization stress model was prepared in the model group using acute immobilization for 2 h. Ginsenoside Rb1 （40 mg/kg） was peritoneally injected to rats in the medication group 30 min before modeling， with the same procedure as those for rats in the model group. No treatment was performed to rats in the normal control group. The plasma adrenocorticotropic hormone （ACTH） and corticosterone （CORT） contents were detected using ELISA. The mRNA expression of TrkB in the rats′ hippocampus was detected using real time fluorescence quantitative RT PCR. Results Before modeling there was no statistical difference of plasma CORT or ACTH concentrations among three groups （P>0.05）. The plasma CORT and ACTH concentrations increased in the model group and the medication group more significantly after modeling than before modeling， showing statistical difference （P<0.05）. Besides， they were obviously higher in the model group than in the normal control group （P<0.05）. They were obviously higher in the medication group than in the model control group （P<0.05）. Compared with the normal control group， the mRNA expression of TrkB significantly decreased in the model group （87.73±7.62 vs 50.65±5.19， P<0.05）， showing statistical difference. The mRNA expression of TrkB was significantly higher in the medication group （78.91±18.07） than in the model group， showing statistical difference （P<0.05）. Conclusion Pretreatment by ginsenoside Rb1 could increase the plasma CORT and ACTH concentrations， maintain the mRNA expression of TrkB， thus relieving injury induced by acute immobilization stress. |
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