| 胡海燕,雷磊,陈翔,王文花.清心开窍方挥发油对AD大鼠大脑皮层及海马区GFAP及Caspase-3表达的影响[J].,2013,33(07):0927-0932 |
| 清心开窍方挥发油对AD大鼠大脑皮层及海马区GFAP及Caspase-3表达的影响 |
| Effects of Qingxin Kaiqiao Recipe Volatile Oil on Expressions of GFAP and Caspase-3 in the Cortex and Hippocampus of AD Rats |
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| DOI：10.7661/CJIM.2013.07.0927 |
| 中文关键词: 阿尔茨海默病 清心开窍方 胶质纤维酸性蛋白 β-淀粉样蛋白 半胱氨酸天门冬氨酸蛋白酶-3 |
| 英文关键词:Alzheimer′s disease Qingxin Kaiqiao Recipe glial fibrillary acidic protein β-amyloid precursor protein cystein-containing aspartate specific protease-3 |
| 基金项目:国家自然科学基金资助项目（No30973780）；浙江省自然科学基金资助项目（No.Y2080273）；温州市科技局资助项目（No.Y20080106） |
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| 中文摘要: |
| 目的探讨清心开窍方及其挥发油对Aβ25－35杏仁核注射诱导痴呆（Alzheimer′s disease， AD）大鼠皮层及海马区胶质纤维酸性蛋白（glial fibrillary acidic protein， GFAP）、β-淀粉样蛋白（β-amyloid， Aβ）、淀粉样前体蛋白（β-amyloid precursor protein，βAPP）及Caspase-3表达的影响。方法选取32只雄性SD大鼠，采用杏仁核注射Aβ25－35诱导AD大鼠模型，造模后随机分为模型组、盐酸多奈哌齐组［盐酸多奈哌齐片，1.67 mg/kg］、清心开窍方组（中药组，清心开窍方煎液12.67 mL/kg）和挥发油组（挥发油，3.33 mL/kg），另选取8只大鼠作为正常组，正常组、模型组给予等量双蒸水灌胃，各组每天干预1次，连续2周。给药结束后进行Morris水迷宫测试，记录各组大鼠水迷宫测试期间第1～5天逃避潜伏期及跨越平台次数。免疫组织化学法检测各组大鼠大脑皮层与海马区GFAP、Aβ、βAPP及Caspase-3的表达。结果与模型组比较，各用药组大鼠第3～5天逃避潜伏期均缩短，皮层与海马区GFAP、Aβ、βAPP及Caspase-3表达水平降低，跨越平台次数增加，差异均有统计学意义（P<0.01，P<0.05）。与西药组比较，中药组皮层及海马区Aβ表达水平降低、βAPP升高，挥发油组皮层及海马区GFAP、βAPP及Caspase-3均升高，挥发油组第3～5天逃避潜伏期明显延长，跨越平台次数减少，差异均有统计学意义（P<0.05，P<0.01）。结论清心开窍方能明显改善AD大鼠学习记忆能力，其机制可能是通过降低皮层及海马区GFAP、Aβ、βAPP及Caspase-3的表达发生作用。 |
| 英文摘要: |
| ObjectiveTo study effects of Qingxin Kaiqiao Recipe （QKR） and its volatile oil on the expressions of Aβ25－35 glial fibrillary acidic protein （GFAP）， β-amyloid （Aβ）， β-amyloid precursor protein （βAPP）， and Caspase-3 in the cortex and hippocampus of Alzheimer′s disease （AD） rats induced by injecting Aβ25－35 into the bilateral amygdala. MethodsTotally 32 male SD rats were selected.The AD rat model was establish by injecting Abeta25－35 from bilateral amygdala. After modeling they were randomly divided into the model group， the Donepezil Hydrochloride group ［Donepezil Hydrochloride Tablet （1.67 mg/kg）， abbreviated as the DH group］， the QKR group （QKR Decoction， 12.67 mL/kg）， and the volatile oil group （3.33 mL/kg）， 8 rats in each group. Another 8 rats were selected as the normal control group. Equal volume of double distilled water was administered to rats in the normal control group and the model group by gastrogavage， once daily for 2 successive weeks. The Morris water maze test was performed by the end of medication. The escape latency and times of crossing the platform in the water maze test were recorded during the 1st day to the fifth day. The expressions of GFAP， Aβ， βAPP， and Caspase-3 in the cortex and hippocampus of the rats in each group were detected by immunohistochemical assay. ResultsCompared with the model group， the escape latency from the 3rd day to the 5th day was shortened， the expressions of GFAP， Aβ， βAPP， and Caspase-3 decreased in the cortex and hippocampus， the times of crossing the platform increased in each medication group， showing statistical difference （P<0.01， P<0.05）. Compared with the DH group， the expressions of Aβ in the cortex and hippocampus decreased， and the βAPP expression increased in the QKR group. The expressions of GFAP， βAPP， and Caspase-3 in the cortex and hippocampus increased in the volatile oil group. The escape latency from the 3rd day to the 5th day was obviously prolonged， and the times of crossing the platform decreased in the volatile oil group， showing statistical difference （P<0.05， P<0.01）. Conclusion QKR could obviously improve the learning and memory capabilities of AD rats， which might be achieved through decreasing the expressions of GFAP， Aβ， βAPP， and Caspase-3 in the cortex and hippocampus. |
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