| 房财富,廖小红,何嘉仑,唐洪梅.肠激安胶囊对IBS-D模型大鼠脑肠轴中NPY mRNA表达及ACTH含量的影响[J].,2013,33(07):0948-9052 |
| 肠激安胶囊对IBS-D模型大鼠脑肠轴中NPY mRNA表达及ACTH含量的影响 |
| Effect of Changji′an Capsule on mRNA Expressions of NPY and ACTH Contents in Brain-Gut Axis of IBS-D Model Rats |
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| DOI：10.7661/CJIM.2013.07.0948 |
| 中文关键词: 肠激安胶囊 腹泻型肠易激综合征 神经肽Y 促肾上腺皮质激素 脑肠轴 |
| 英文关键词:Changji′an Capsule diarrhea predominant irritable bowel syndrome neuropeptide Y adreno-cortico-tropic hormone brain-gut axis |
| 基金项目:国家自然科学基金资助项目 （No81073160）； 广东省自然科学基金资助项目 （No10151040701000041，No.S2012010010539） |
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| 中文摘要: |
| 目的探讨肠激安胶囊对腹泻型肠易激综合征（diarrhea predominant irritable bowel syndrome，IBS-D）大鼠下丘脑和结肠组织神经肽Y（neuropeptide Y，NPY）mRNA表达和血清促肾上腺皮质激素（adreno-cortico-tropic hormone，ACTH）含量的影响。方法将SD大鼠随机分为正常组，模型组，匹维溴铵组（0.018 g/kg），肠激安胶囊高（2.812 g/kg）、中（1.406 g/kg）、低（0.703 g/kg）剂量组，每组8只，模型组以及给药组采用母乳分离+醋酸刺激+四肢束缚法制备IBS-D大鼠模型，正常组和模型组给予生理盐水，给药组灌胃14天，采用ELISA法检测各组大鼠血清ACTH含量，采用RT-PCR法检测各组大鼠下丘脑及结肠组织 NPY mRNA 表达。结果与正常组比较，模型对照组大鼠血ACTH明显升高（P<0.01），下丘脑、结肠组织NPY mRNA表达明显降低（P<0.01）；与模型组比较，肠激安胶囊高、中剂量组及匹维溴胺组大鼠血ACTH 明显降低（P<0.01，P<0.05），匹维溴铵组和肠激安胶囊高、中、低剂量组下丘脑、结肠组织NPY mRNA表达上调（P<0.05）。结论肠激安胶囊对IBS-D大鼠脑肠轴异常有调节作用， 其作用机制可能是通过上调下丘脑和结肠组织NPY mRNA表达和下调HPA轴中ACTH含量。 |
| 英文摘要: |
| ObjectiveTo explore the effect of Changji′an Capsule （CA） on mRNA expressions of neuropeptide Y （NPY） in the hypothalamus and colon and serum levels of adreno-cortico-tropic hormone （ACTH） in rats of diarrhea predominant irritable bowel syndrome （IBS-D） model rats. MethodsTotally 48 SD rats were randomly divided into six groups， i.e.， the normal control group， the model group， the Pinaverium Bromide group （PB， 0.018 g/kg）， the high dose CA group （2.812 g/kg）， the medium dose CA group （1.406 g/kg）， and the low dose CA group （0.703 g/kg）， 8 in each group. The IBS-D rat model was established by using separation of breast milk + stimulation of acetic acid + constraint of four limbs. Normal saline was given to rats in the normal control group and the model group. All medication lasted for 14 successive days by gastrogavage. The serum content of ACTH was detected by enzyme linked immunosorbent assay （ELISA）. The expressions of NPY mRNA in the colon and the hypothalamus were detected using real-time fluorescence quantitative PCR. ResultsCompared with the normal control group， the serum ACTH content significantly increased （P<0.01）， the NPY mRNA expression in the colon and the hypothalamus obviously decreased （P<0.01） in the model control group. Compared with the model group， the serum ACTH obviously decreased in the high dose CA group， the medium dose CA group， and the PB group （P<0.01， P<0.05）. The NPY mRNA expression in the colon and the hypothalamus were obviously up-regulated in the high dose CA group， the medium dose CA group， the low dose CA group， and the PB group （P<0.05）. ConclusionsCA could modulate the abnormity of brain-gut axis of IBS-D rats possibly by up-regulating NPY mRNA expressions in the hypothalamus and the colon and down-regulating the ACTH content in the hypothalamic-pituitary-adrenal axis. |
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