| 潘爱珍,董霄,项婷,张诗军.高脂及劳倦伤脾大鼠有机阴离子转运肽oatp2b1 mRNA及蛋白表达研究[J].,2013,33(07):0953-0957 |
| 高脂及劳倦伤脾大鼠有机阴离子转运肽oatp2b1 mRNA及蛋白表达研究 |
| Study on mRNA and Protein Expressions of Organic Anion Transporting Polypeptide （oatp2b1） in Rats with High Fat Diet and Overstrain Induced Pi Deficiency Syndrome |
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| DOI：10.7661/CJIM.2013.07.0953 |
| 中文关键词: 湿浊 伤脾 有机阴离子转运肽2b1 |
| 英文关键词:damp impairment of Pi organic anion transporting polypeptide 2b1 |
| 基金项目:国家自然科学基金资助项目（No81072806）；国家“十一五”科技重大专项（No2009ZX10005－016）；广东省自然科学基金资助项目（No9151008004000030） |
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| 中文摘要: |
| 目的观察脾虚状态下大鼠有机阴离子转运肽oatp2b1基因和蛋白表达，探讨oatp2b1在湿浊转运中的作用。方法24只大鼠随机分为正常组、高脂组、劳倦组，每组8只。造模成功后，每只大鼠取肺、脾、肝、肾、胃、小肠、大肠组织各1块。劳倦组单号日期采用特制束缚筒，每次3 h，双号日期令大鼠在冷水（10±1） ℃中游泳7 min，连续12周。模型组、正常组常规标准大鼠颗粒饲料喂养12周，高脂组大鼠以高脂饲料喂养12周，均自由饮食饮水。采用RT-PCR、Western Blot法检测肺、脾、肝、肾、胃、小肠、大肠组织oatp2b1 mRNA、蛋白表达。结果高脂组肾、肝oatp2b1 mRNA含量高于正常组及劳倦组（P<0.01， P<0.05）；正常组oatp2b1 mRNA表达量由高至低为肝>肺>脾>大肠>小肠>肾>胃；劳倦组oatp2b1 mRNA表达量由高至低为肝>肺>大肠>脾>肾>胃>小肠；高脂组oatp2b1 mRNA表达量由高至低为肝>肺>脾>小肠>肾>大肠>胃。肺组织oatp2b1蛋白表达量由高至低为劳倦组>正常组>高脂组（P>0.05）。脾组织oatp2b1蛋白表达由高至低为高脂组>正常组>劳倦组（P>0.05）；肾组织oatp2b1蛋白表达量由高至低为正常组>劳倦组>高脂组（P>0.05）；肝组织oatp2b1蛋白表达量由高至低为正常组>高脂组>劳倦组（P>0.05）；其中胃、大肠、小肠中oatp2b1蛋白表达量极低。正常组oatp2b1蛋白表达由高至低为肺>脾>肝，肾>胃、大肠、小肠；劳倦组oatp2b1蛋白表达由高至低为：肺>脾>肾>肝>胃、大肠、小肠；高脂组oatp2b1蛋白表达由高至低为：脾>肺>肾>肝>胃，大肠，小肠，差异无统计学意义（P>0.05）。结论肝、肾等脏器在脾失健运状态下的湿浊转运中可能发挥重要作用，oatp2b1是机体参与湿浊运化的物质基础之一。脾主运化湿浊的功能，可能不仅包括胃肠道的功能在内，而且也可能包括一部分肝脏及肾脏的功能。 |
| 英文摘要: |
| ObjectiveTo explore roles of mRNA and protein expressions of organic anion transporting polypeptide （oatp2b1） of rats with high fat diet and overstrain induced Pi deficiency syndrome in the transporting of damp turbidity. MethodsTotally 24 SD rats were randomly divided into three groups， i.e.， the normal group， the overstrain group， and the high fat diet group， 8 in each group. After successful modeling， one piece of tissues such as spleen， kidney， liver， lung， stomach， small intestine， and large intestine was taken from each rat. Rats of the overstrain group were bonded by specially made bondage cylinder， 3 h each time on odd days， and forced to swim in cold water （10±1） ℃ for 7 min on even days alternatively for twelve weeks. Rats in the model group and the normal group were fed with standard routine granular forage for 12 weeks. Rats in the high fat diet group were fed with high fat forage for twelve weeks. All rats drank and ate freely. The mRNA and protein expressions of oatp2b1 were detected in the seven tissues using RT-PCR and Western blot. ResultsThe mRNA expression of oatp2b1 in liver and kidney tissues of rats in the high fat diet group was higher when compared with that of the normal group and the overstrain group （P<0.01， P<0.05）. The oatp2b1 mRNA expression in the normal group was sequenced from high to low as liver>lung>spleen>larger intestine>small intestine>kidney>stomach. The oatp2b1 mRNA expression in the overstrain group was sequenced from high to low as liver>lung>larger intestine>spleen>kidney>stomach>small intestine. The oatp2b1 mRNA expression in the high fat diet group was sequenced from high to low as liver>lung>spleen>small intestine>kidney>larger intestine>stomach. The oatp2b1 protein expression in the lung tissue was sequenced from high to low as the overstrain group>the normal group>the high fat diet group （P>0.05）. The oatp2b1 protein expression in the spleen tissue was sequenced from high to low as the high fat diet group>the normal group>the overstrain group （P>0.05）. The oatp2b1 protein expression in the kidney tissue was sequenced from high to low as the normal group>the overstrain group>the high fat diet group （P>0.05）. The oatp2b1 protein expression in the liver tissue was sequenced from high to low as the normal group>the high fat diet group>the overstrain group （P>0.05）. Of them， the oatp2b1 protein expressed extremely less in the stomach， large intestine， and small intestine. The oatp2b1 protein expression in the normal group was sequenced from high to low as lung>spleen>liver， kidney>stomach， larger intestine， and small intestine. The oatp2b1 protein expression in the overstrain group was sequenced from high to low as lung>spleen>kidney>liver>stomach， larger intestine， and small intestine. The oatp2b1 protein expression in the high fat diet group was sequenced from high to low as spleen>lung>kidney>liver>stomach， larger intestine， and small intestine. However， there was no statistical significance among the three groups by pair-wise comparison （P>0.05）. ConclusionsKidney and liver might play important roles in the transportation and transformation of damp under the state of Pi deficiency syndrome. Oatp2b1 may be one of the material bases involved in the transportation and transformation of damp turbidity. Pi′s function of governing transportation and transformation of damp might not only include the functions of the gastrointestinal tract， but also include partial liver and kidney functions. |
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