| 蒋莉娅,赵弘卿,诸静芬,黄继人.针刺对重症急性胰腺炎早期急性肺损伤大鼠血清MIP-2蛋白及肺与大肠组织MIP-2 mRNA表达的影响[J].,2013,33(07):0958-0962 |
| 针刺对重症急性胰腺炎早期急性肺损伤大鼠血清MIP-2蛋白及肺与大肠组织MIP-2 mRNA表达的影响 |
| Effect of Acupuncture on Serum MIP-2 and MIP-2 mRNA Expressions in Isolated Fei and Dachang of Severe Acute Pancreatitis Induced Acute Lung Injury Rats in the Acute Phase |
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| DOI：10.7661/CJIM.2013.07.0958 |
| 中文关键词: 针刺 胰腺炎 急性肺损伤 肺 大肠 巨噬细胞炎症蛋白-2 |
| 英文关键词:acupuncture pancreatitis acute lung Injury Fei Dachang macrophage inflammatory protein-2 |
| 基金项目:国家重点基础研究发展计划（973计划）资助项目（No2009CB522702） |
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| 中文摘要: |
| 目的观察针刺对重症急性胰腺炎（severe acute pancreatitis， SAP）早期急性肺损伤（acute lung injury， ALI）大鼠血清巨噬细胞炎症-2（macrophage inflammatory protein-2， MIP-2）蛋白及肺与大肠组织MIP-2 mRNA表达的影响。方法40只雄性Wistar大鼠随机分为假手术组、SAP组、针刺治疗组和对照组，每组10只。以3.5%牛黄胆酸钠逆行注射胰胆管的方法制作SAP模型，针刺治疗组在“肺与大肠相表里”的理论指导下，于SAP造模0.5 h后行针刺肺、大肠、脾经穴位及其背腧穴；针刺对照组在造模0.5 h后行针刺肺、脾经穴位及其背腧穴。观察造模6 h后各组大鼠血清肿瘤坏死因子（tumor necrosis factor alfa， TNF-α）、一氧化氮（nitric oxide， NO）及MIP-2蛋白表达水平，应用RT-PCR法检测大鼠肺与大肠组织MIP-2 mRNA表达水平。结果SAP组6 h后血清TNF-α、NO、MIP-2蛋白表达水平及肺与大肠组织MIP-2 mRNA表达水平均明显高于假手术组（P<0.05），针刺治疗组各指标均低于SAP组及针刺对照组（P<0.05）。针刺治疗组、SAP组及针刺对照组大鼠MIP-2蛋白及mRNA间均呈正相关（P<0.05）。结论“肺与大肠相表里”理论指导下的针刺对SAP大鼠早期急性肺损伤保护作用明显，其作用机制可能与通过抑制肺与大肠组织MIP-2 mRNA的过度表达，降低血清MIP-2蛋白表达水平有关。 |
| 英文摘要: |
| ObjectiveTo observe effect of acupuncture on serum macrophage inflammatory protein-2 （MIP-2） and MIP-2 mRNA expressions in isolated Fei and Dachang of severe acute pancreatitis （SAP） induced acute lung injury （ALI） rats in the acute phase. MethodsForty male Wistar rats were randomly divided into four groups， i.e.， the sham-operation group， the SAP group， the acupuncture treatment group， and the acupuncture control group， 10 in each group. The SAP model was induced by retrograde infusion of 3.5% sodium taurocholate into the pancreatobiliary duct. Under the guidance of “Fei and Dachang exterior-interiorly related”， points were acupunctured along Fei， Dachang， and Pi channels， as well as those points on the back of rats in the acupuncture treatment group 0.5 h after modeling. Besides， points were acupunctured along Fei and Pi channels， as well as those points on the back of rats in the acupuncture control group 0.5 h after modeling. Serum levels of tumor necrosis factor α （TNF-α） and nitric oxide（NO）， and MIP-2 expressions were examined 6 h after modeling. Expressions of MIP-2 mRNA in isolated lung and large intestine tissues were detected by reverse transcription PCR. ResultsCompared with the sham-operation group， serum levels of TNF-α and NO， and expressions of MIP-2 and MIP-2 mRNA in isolated lung and large intestine tissues were significantly higher in the SAP group （P<0.05）. Each index was lower in the acupuncture treatment group than in the SAP group and the acupuncture control group （P<0.05）. Besides， the serum level of MIP-2 and the MIP-2 mRNA expression in isolated lung and large intestine tissues were positively correlated in all groups except the sham-operation group （P<0.05）. ConclusionsUnder the guidance of “Fei and Dachang exterior-interiorly related”， acupuncture could remarkably reduce the severity of SAP induced ALI rats in the acute phase. Its mechanism might be related to suppressing over-expressions of MIP-2 mRNA in isolated lung and large intestine tissues， and lowering the serum MIP-2 expression level. |
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