| 蔡东焱,高翔,吴小红,洪婷婷.β-榄香烯注射液联合紫杉醇注射液对乳腺癌MB-468细胞体外协同作用研究[J].,2013,33(07):0978-0982 |
| β-榄香烯注射液联合紫杉醇注射液对乳腺癌MB-468细胞体外协同作用研究 |
| Synergistic Effect of β-elemene Injection Combined Paclitaxel Injection on Human Breast Cancer MB-468 Cells： an in vitro Study |
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| DOI：10.7661/CJIM.2013.07.0978 |
| 中文关键词: 乳腺癌 β-榄香烯 紫杉醇 协同作用 细胞周期 |
| 英文关键词:breast cancer β-elemene Paclitaxel synergistic effect cell cycleβ |
| 基金项目:无锡市医管中心科技计划项目（No.ygm1017） |
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| 中文摘要: |
| 目的探讨β-榄香烯与紫杉醇对乳腺癌细胞株协同作用及其机制。方法体外分别以β-榄香烯注射液（2.5、5.0、10.0、20.0、40.0、80.0、160.0、320.0、640.0 μg/mL）、紫杉醇注射液（0.00100、0.00200、0.00400、0.00800、0.01600、0.03125、0.06250、0.12500、0.25000 μg/mL） 作用24 h和48 h以及两药联合处理人乳腺癌细胞株MB-468细胞，SRB法检测增殖变化，流式细胞仪检测细胞凋亡和细胞周期分布，通过 Western blot 检测干预后细胞周期蛋白依赖性激酶1（cyclin-dependent kinase，CDK1）、细胞周期蛋白B1（cyclin B1）、细胞周期调控蛋白P21 cip1和P27 kip1表达变化。结果单药β-榄香烯或紫杉醇对MB-468细胞株生长均有抑制作用，β-榄香烯24 h和48 h的IC50和IC20值分别为34.20、52.59和10.15、17.81 μg/mL，紫杉醇24、48 h的IC50值分别为2.449和1.698 μg/mL。联合组（β-榄香烯和紫杉醇的浓度分别为20、40和0.016、0.008 μg/mL）对乳腺癌MB-468细胞株的生长抑制率具有协同作用，Q值>1.15。β-榄香烯组（52.59 μg/mL）cyclin-B1蛋白表达有明显的下降， 联合组较紫杉醇单药组（1.698 μg/mL）cyclin B1的表达水平也有降低，联合组P27 kip1蛋白的表达较β-榄香烯和紫杉醇单药作用时有增加。结论β-榄香烯对紫杉醇具有协同作用，其可能机制是通过下调细胞周期蛋白cyclin B1表达和上调P27 kip1表达有关。 |
| 英文摘要: |
| ObjectiveTo observe the synergistic effect of β-elemene Injection （βI） combined Paclitaxel Injection （PI） on breast cancer MB-468 cells and to study possible mechanisms. MethodsBreast cancer MB-468 cells were treated with βI （2.5， 5.0， 10.0， 20.0， 40.0， 80.0， 160.0， 320.0， and 640.0 μg/mL）， PI （0.00100， 0.00200， 0.00400， 0.00800， 0.01600， 0.03125， 0.06250， 0.12500， and 0.25000 μg/mL）， and βI combined PI for 24 h and 48 h respectively. Cell proliferation was determined using SRB assay. Cell apoptosis and cell cycle phase distribution were detected using flow cytometry. The post-intervention expressions of cell cycle proteins ［cyclin-dependent kinase （CDK1）， cyclin-B1， P21cip1， and P27kip1］ were detected by Western blot. Resultsβ-elemene or paclitaxel inhibited the growth of MB-468 cell line. The IC50 and IC20 values treated with β-elemene for 24 h were 34.20 and 52.59 μg/mL and for 48 h were 10.15 and 17.81 μg/mL respectively， while the IC50 values treated with paclitaxel for 24 h and 48 h were 2.449 and 1.698 μg/mL respectively. β-elemene （20 and 40 μg/mL respectively） and Paclitaxel （0.016 and 0.008 μg/mL respectively） synergistically inhibited cell proliferation of MB-468 cells， with Q value>1.15. β-elemene alone （52.59 μg/mL） apparently decreased the expression of cyclin-B1 protein. The expression of cyclin-B1 protein in the combined group was also lower than that in the PI group （1.698 μg/mL）. The expression of P27kip1 was up-regulated when compared with that in the βI group or the PI group. Conclusionβ-elemene had synergistic effect with Paclitaxel， and its possible mechanism might be correlated with down-regulating the cell cycle protein cyclin-B1 expression and up-regulating the P27kip1 expression. |
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