| 李继君,汤海霞,尹文静,李安源.宁动颗粒对不同中医证型抽动－秽语综合征模型大鼠刻板行为的影响[J].,2013,33(08):1112-1115 |
| 宁动颗粒对不同中医证型抽动－秽语综合征模型大鼠刻板行为的影响 |
| Effect of Ningdong Granule on Stereotyped Behaviors in Tourette Syndrome Model Rats of Different Chinese Medical Syndromes |
| |
| DOI：10.7661/CJIM.2013.08.1112 |
| 中文关键词: 宁动颗粒 抽动－秽语综合征 中医证型 刻板行为 |
| 英文关键词:Ningdong Granule Tourette′s syndrome Chinese medical syndrome stereotyped behavior |
| 基金项目:国家自然科学基金资助项目（No. 81202724） |
|
| Hits: 2689 |
| Download times: 1729 |
| 中文摘要: |
| 目的 观察宁动颗粒对不同中医证型抽动－秽语综合征（Tourette′s syndrome，TS）大鼠模型刻板行为的影响。方法 32只Wistar大鼠分别以心肝亏虚证、肝肾阴虚证、痰瘀阻窍证、脾虚肝亢证TS患儿血清诱导建立相应证型TS大鼠模型，每组8只，血清微量灌注同时给予宁动颗粒120 mg/kg灌胃，每天3次，连续3周；另设正常对照组（8只），注射健康儿童血清，同时给予生理盐水灌胃。分别在给药第1、7、14、21天记录各组大鼠刻板行为次数。结果 TS患儿血清抗神经抗体浓度高于健康儿童血清［U/L，（1.28±0.36） vs （0.52±0.24）］，差异有统计学意义（P<0.01）；心肝亏虚证、肝肾阴虚证、痰瘀阻窍证、脾虚肝亢证TS患儿血清抗神经抗体浓度分别为（1.34±0.41）、（1.19±0.51）、（1.29±0.61）、（1.17±0.45）U/L，不同证型比较，差异无统计学意义（P>0.05）。给药前4个证型组大鼠刻板行为比较，差异无统计学意义（P>0.05）；除脾虚肝亢证组给药第14天外，心肝亏虚证组在给药第7、14、21天大鼠刻板行为次数均显著低于同期肝肾阴虚证组、痰瘀阻窍证组及脾虚肝亢证组，差异均有统计学意义（P<0.05， P<0.01）。给药21天后，心肝亏虚证组刻板行为总计数改善情况（42.8±12.6）明显优于肝肾阴虚证组（29.3±13.7）、痰瘀阻窍证组（21.9±10.4）、脾虚肝亢证组（30.6±9.6），差异均有统计学意义（P<0.01， P<0.05）。结论 TS患儿血清中抗神经抗体浓度明显高于健康儿童，富含抗神经抗体TS血清可以诱导出刻板行为大鼠模型；宁动颗粒对心肝亏虚证TS模型大鼠刻板行为的改善优于其他证型。 |
| 英文摘要: |
| Objective To observe the effect of Ningdong Granule （NDG） on stereotyped behaviors in Tourette′s syndrome （TS） model rats of different Chinese medical syndromes. Methods Thirty-two Wistar rats were used to establish TS models of different Chinese medical syndromes （n=8） induced by TS children patients′ sera of 4 syndromes， i.e.， Xin-Gan deficiency syndrome （XGDS）， Gan-Shen yin deficiency syndrome （GSYDS）， sputum-turbid blocking aperture syndrome （STBAS）， and Gan hyperactivity Pi deficiency syndrome （GHPDS）. Corresponding sera was micro-infused to them while administering NDG （120 mg/kg each time， thrice daily， for 3 successive weeks）. Besides， another normal control group （n=8） was set up by injecting sera from healthy children plus intragastric perfusion of normal saline. Stereotyped behaviors were recorded on the 1st， 7th， 14th， and 21st day after administration of NDG. Results The anti-neural antibody serum concentration in TS children was significantly higher than that in healthy control ［（1.28±0.36） U/L vs （0.52±0.24） U/L， P<0.01］. It was （1.34±0.41） U/L in the XGDS group， （1.19±0.51） U/L in the GSYDS group， （1.29±0.61） U/L in the STBAS group， and （1.17±0.45） U/L in the GHPDS group， showing no statistical difference （P>0.05）. There was no statistical difference in stereotypic behaviors of rats after treatment among the four different Chinese medical syndromes （P>0.05）. At day 7， 14， and 21 after treatment by NDG， the times of stereotyped behaviors were significantly less in the XGDS group than in the other three groups at the same time points except in the GHPDS group at day 14 （P<0.05， P<0.01）. Meanwhile， the total numbers of stereotyped behaviors in the XGDS group ［（42.8±12.6）］ was obviously superior to that in the GSYDS group ［（29.3±13.7）］， the STBAS group ［（21.9±10.4）］， and the GHPDS group ［（30.6±9.6）］， showing statistical difference （P<0.01， P<0.05） after treatment by NDG at day 21. Conclusions The anti-neural antibody serum concentration in TS children was significantly higher than that in healthy children. Stereotyped behaviors could be induced in rats after intrastriatal micro-infusion of TS sera rich in anti-neural antibody. TS model rats of XGDS were better improved than rats in the other 3 groups after treatment by NDG. |
| View Full Text View/Add Comment Download reader |
