| 乐薇,肖颖,田佳玉,陈盈芳.电针丰隆穴对高脂血症模型大鼠巨噬细胞炎症因子表达的影响[J].,2013,33(10):1361-1366 |
| 电针丰隆穴对高脂血症模型大鼠巨噬细胞炎症因子表达的影响 |
| Effect of Electroacupuncture at Fenglong （ST40） on Expressions of Inflammatory Factors in Macrophages of Hyperlipidemia Model Rats |
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| DOI：10.7661/CJIM.2013.10.1361 |
| 中文关键词: 电针 丰隆穴 高脂血症 巨噬细胞 细胞间黏附分子-1 单核细胞趋化蛋白-1 肿瘤坏死因子-α 白细胞介素-6 |
| 英文关键词:electroacupuncture Fenglong （ST40） hyperlipemia macrophage intercellular adhesion molecule-1 monocyte chemoattractant protein 1 tumor necrosis factor-α interleukin 6 |
| 基金项目:武汉市卫生局科研项目 （No.WZ11B01） |
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| 中文摘要: |
| 目的 观察电针丰隆穴对高脂血症大鼠巨噬细胞炎症因子的影响，探讨电针在治疗高脂血症中的作用。方法 选取成年雄性SD大鼠50只，适应性喂养1周后，将实验大鼠随机分为空白组、模型组、饮控组、电针组、饮控加电针组5组，每组10只。治疗结束后，将各组大鼠经颈动脉取血，测定血清总胆固醇（TC）、血清甘油三酯（TG）、高密度脂蛋白（HDL-C）、低密度脂蛋白（LDL-C）的含量；再分离大鼠腹腔巨噬细胞，加荧光标记的炎症因子分子抗体后应用流式细胞仪检测细胞间黏附分子－1（ICAM-1）、单核细胞趋化蛋白－1（MCP-1）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）的含量。结果 与空白组比较，模型组大鼠血清TC、LDL-C明显上升（P<0.01），HDL-C明显下降（P<0.01），而TG变化并不明显（P>0.05）；与模型组比较，饮控组、电针组及饮控加电针组大鼠血清TC、LDL-C明显下降（P<0.01）；与饮控组比较，电针组及饮控加电针组大鼠血清中TC、LDL-C降低明显（P<0.01）。与空白组比较，模型组巨噬细胞含量增加显著（P<0.01）；与模型组比较，电针组和饮控加电针组巨噬细胞含量均明显下降，以饮控加电针组为甚（P<0.01）；与饮控组比较，饮控加电针组大鼠巨噬细胞含量明显下降（P<0.05）。与空白组比较，模型组大鼠腹腔巨噬细胞内ICAM-1、MCP-1、TNF-α、IL-6均有显著升高（P<0.01）。与模型组比较，饮控组、电针组、饮控加电针组各组ICAM-1、MCP-1、TNF-α、IL-6值均下降，但以饮控加电针组下降更为显著（P<0.01）；与饮控组比较，饮控加电针组IL-6、ICAM-1、MCP-1、TNF-α均有显著下降（P<0.05， P<0.01）。结论 电针丰隆穴能够明显下调高脂血症大鼠血清中的TC、LDL-C水平，减少大鼠巨噬细胞及其炎症因子ICAM-1、MCP-1、TNF-α、IL-6的含量，从而达到对高脂血症的治疗作用。 |
| 英文摘要: |
| Objective To observe the effect of electroacupuncture （EA） at Fenglong （ST40） on inflammatory factors in macrophages of hyperlipemia （HLP） rats， and to explore the role of EA in treating HLP. Methods Totally 50 adult male SD rats were selected. After one-week adaptative feeding， they were randomly divided into 5 groups， i.e.， the blank control group （Group A）， the model group （Group B）， the diet control group （Group C）， the EA group （Group D）， the diet control+EA group （Group E）， 10 in each group. By the end of treatment， all rats were sacrificed to get blood from the carotid artery. The contents of serum total cholesterol （TC）， triglyceride （TG）， high density lipoprotein cholesterol （HDL-C）， and low density lipoprotein cholesterol （LDL-C） were determined. Peritoneal macrophages were isolated. Contents of intercellular adhesion molecule-1 （ICAM-1）， monocyte chemoattractant protein 1 （MCP-1）， tumor necrosis factor-α （TNF-α）， and interleukin 6 （IL-6） were detected using flow cytometry （FCM） after adding the fluorescein-labeled molecule antibodies of inflammatory factors. Results Compared with Group A， the serum contents of TC and LDL-C obviously increased and HDL-C significantly decreased in Group B （all P<0.01）. There was no obvious change in the content of TG between Group A and Group B （P>0.05）. Compared with Group B， the contents of TC and LDL-C significantly decreased in Group C， D， and E （P<0.01）. Compared with Group C， serum contents of TC and LDL-C obviously decreased in Group D and E （P<0.01）. Compared with Group B， the content of macrophages significantly increased in Group A （P<0.01）. Compared with Group B， the content of macrophages significantly decreased in Group D and E （P<0.01）， more obviously in Group E （P<0.01）. Compared with Group C， the content of macrophages significantly decreased in Group E （P<0.05）. Compared with Group A， levels of ICAM-1， MCP-1， TNF-α， and IL-6 in the peritoneal macrophages significantly increased in Group B （P<0.01）.Compared with Group B， levels of ICAM-1， MCP-1， TNF-α， and IL-6 significantly decreased in Group C， D， and E， but more obviously in Group E （P<0.01）. Compared with Group C， levels of ICAM-1， MCP-1， TNF-α （P<0.05， P<0.01）， and IL-6 （P<0.05） significantly decreased in Group E. Conclusion EA at Fenglong （ST40） could obviously down-regulate the serum levels of TC and LDL-C in HLP rats， reduce the contents of macrophages， ICAM-1， MCP-1， TNF-α， and IL-6， thus arriving at the therapeutic effect in treating HLP. |
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