| 梁宜,房军帆,杜俊英,方剑乔.电针对CFA大鼠脊髓背角NR2B酪氨酸1472位点磷酸化的影响[J].,2013,33(10):1372-1375 |
| 电针对CFA大鼠脊髓背角NR2B酪氨酸1472位点磷酸化的影响 |
| Effect of Electroacupuncture on Phosphorylation of NR2B at Tyr 1742 Site in the Spinal Dorsal Horn of CFA Rats |
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| DOI：10.7661/CJIM.2013.10.1372 |
| 中文关键词: 电针 炎症性疼痛 NR2B磷酸化 |
| 英文关键词:electroacupuncture inflammatory pain phosphorylation of NR2B |
| 基金项目:国家自然科学基金资助项目（No.30873305）；浙江省教育厅科研项目（No.Y200805538）；浙江中医药大学附属第三医院科研课题（No.ZS10CA01）；国家中医药管理局中医药重点学科（针灸学）建设经费资助（No.国中医药发［2009］30号） |
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| 中文摘要: |
| 目的 观察电针对完全福氏佐剂（CFA）致炎症性疼痛模型大鼠脊髓NR2B酪氨酸1472位点磷酸化的影响。方法 40只雄性SD大鼠随机分为正常对照组（N组，10只）、模型对照组（CFA组，15只）和电针治疗组（EA组，15只）。采用右后足底皮下注射CFA（0.1 mL/只），建立炎性痛大鼠模型。分别观察造模前、造模后24、25 h，3、7 d 5个时点的痛阈变化，并采用免疫组化法检测造模后3 d时大鼠患侧脊髓背角NR2B酪氨酸1472位点磷酸化表达。结果 于造模后各时点，CFA组大鼠缩腿阈（PWTs）均低于同期N组（P<0.01）；EA组大鼠PWTs于造模后24 h时明显低于同期N组（P<0.01），接受电针治疗后PWTs呈现升高趋势，尤以造模后25 h和3 d两个时点显著高于同期CFA组（P<0.01）。 造模后3 d时，与同期N组比较，CFA组大鼠患侧脊髓背角浅层p NR2B阳性细胞率有明显升高趋势；与CFA组比较，EA组大鼠患侧脊髓背角p NR2B阳性细胞率呈下降趋势。结论 电针可有效对抗CFA诱导的炎症性疼痛，可能与其下调患侧脊髓背角NR2B酪氨酸1742位点磷酸化相关。 |
| 英文摘要: |
| Objective To observe the effect of electroacupuncture （EA） on phosphorylation of spinal NR2B at Tyr 1742 site in complete Freund′s adjuvant （CFA） induced inflammatory pain rats. Methods Forty male Sprague Dawley rats were randomly divided into normal group （N group， n=10）， the model group （CFA group， n=15）， and the EA group （n=15）. The inflammatory pain model was established by subcutaneous injecting CFA （0.1 mL per rat） into the right hind paw. Paw withdrawal thresholds （PWTs） were measured before CFA injection （as the base）， as well as at 24 h， 25 h， 3rd day， and 7th day after CFA injection. Phosphorylation of NR2B at Tyr 1742 site in the ispilateral spinal dorsal horn at the 3rd day post injection were detected using immunohistochemical assay. Results PWTs in the CFA group were significantly lower than those of the N group at every detective time point post injection （P<0.01）. PWTs were obviously lower in the EA group than in the N group at 24 h post injection （P<0.01）. It showed increasing tendency， markedly higher than those of the CFA group at 25 h and 3rd day post injection （P<0.01）. Compared with the N group， the ratio of p NR2B positive cells in the ispilateral spinal dorsal horn of rats in the CFA group was up regulated. Compared with the CFA group， the ratio of p NR2B positive cells in the ispilateral spinal dorsal horn of rats showed a decreasing tendency in the EA group. Conclusion EA might effectively inhibit CFA induced inflammatory pain possibly associated with down regulating phosphorylation of NR2B at Tyr 1742 site in the ispilateral spinal dorsal horn. |
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