| 高燕,潘超,左芳芳,肖长虹.三水白虎汤对滑膜成纤维细胞增殖及IL-6、IL-17分泌的影响[J].,2013,33(10):1385-1388 |
| 三水白虎汤对滑膜成纤维细胞增殖及IL-6、IL-17分泌的影响 |
| Effect of Sanshui Baihu Decoction on the Proliferation of Fibroblast like Synoviocytes and Its Secretion of IL-6 and IL-17 |
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| DOI：10.7661/CJIM.2013.10.1385 |
| 中文关键词: 三水白虎汤 滑膜成纤维细胞 增殖 白细胞介素-17 |
| 英文关键词:Sanshui Baihu Decoction fibroblast-like synoviocyte proliferation interleukin-17 |
| 基金项目:国家自然科学基金资助项目（No81072936） |
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| 中文摘要: |
| 目的 观察三水白虎汤含药血清对体外培养的类风湿关节炎（RA）、骨关节炎（OA）滑膜成纤维细胞（FLS）增殖及分泌白细胞介素-6（IL-6）、IL-17的影响，探讨三水白虎汤的作用机制。方法 组织块培养法收集RA、OA患者的关节FLS，体外培养传代，取3～6代细胞，加入20%三水白虎汤含药血清培养72 h，MTT法连续检测FLS的增殖率，ELISA法检测上清液中IL-6、IL-17的水平，以来氟米特和生理盐水含药血清作对照。结果 三水白虎汤含药血清对RA、OA FLS增殖有显著抑制作用，同时还能显著抑制RA-FLS分泌IL-17，抑制效率与来氟米特相当；但对RA-FLS的IL-6分泌无影响。对OA-FLS的IL-17、IL-6的分泌均无显著影响。结论 复方中药三水白虎汤能抑制FLS的增殖，并能抑制RA-FLS分泌IL-17，这可能是该方治疗RA的药理机制之一。 |
| 英文摘要: |
| Objective To observe the effect of Sanshui Baihu Decoction （SBD） containing serum on the proliferation of in vitro cultured fibroblast-like synoviocytes （FLS）derived from rheumatoid arthritis （RA） and osteoarthritis （OA） and its secretion of interleukin-6 （IL-6） and IL-17， and to explore the pharmacological mechanism of SBD. Methods The FLS obtained from cultured RA and OA patients′ synovial tissue were cultured and passaged in vitro in a routine way. The cultured medium was changed to DMEM with 20% SBD containing serum and cultured for 72 h after cultured for 3 to 6 generations. The proliferation rate of FLS was detected by MTT assay. Levels of IL-6 and IL-17 in the supernatant were detected by ELISA. Leflunomide and saline containing serum were used as positive and negative control respectively. Results SBD containing serum significantly inhibited the proliferation of RA-FLS and OA-FLS， and decreased the secretion of IL-17 in RA-FLS. Its inhibition efficiency of SBD was equivalent to that of Leflunomide. No obvious inhibition on the secretion of IL-6 in RA-FLS was observed. It had no significant effect on the secretion of IL-17 and IL-6 in OA-FLS. Conclusion SBD could inhibit the proliferation of FLS and the secretion of IL-17 in RA-FLS， which might be one of its pharmacological mechanisms for treating RA. |
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