| 余海,吴萌,谢晶,卢芙蓉.葫芦巴4－羟基异亮氨酸对高糖诱导小鼠3T3-L1脂肪细胞胰岛素抵抗的影响[J].,2013,33(10):1394-1399 |
| 葫芦巴4－羟基异亮氨酸对高糖诱导小鼠3T3-L1脂肪细胞胰岛素抵抗的影响 |
| Effect of Trigonella Foenum graecum 4-Hydroxyisoleucine on High-glucose Induced Insulin Resistance in 3T3-L1 Adipocytes of Mice |
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| DOI：10.7661/CJIM.2013.10.1394 |
| 中文关键词: 葫芦巴 4－羟基异亮氨酸 胰岛素抵抗 肿瘤坏死因子-α |
| 英文关键词:Trigonella Foenum-graecum L. 4-hydroxyisoleucine insulin resistance tumor necrosis factor-α |
| 基金项目:国家自然科学基金资助项目（No81001670，81202962）；华中科技大学同济医学院附属协和医院院内基金项目（No2010－17） |
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| 中文摘要: |
| 目的 研究葫芦巴活性成分4－羟基异亮氨酸（4-hydroxyisoleucine， 4-HIL）对高糖诱导的小鼠前脂肪细胞（3T3-L1）胰岛素抵抗的作用及其机制。方法 以25 mmol/L葡萄糖加0.6 nmol/L胰岛素诱导小鼠3T3-L1脂肪细胞建立胰岛素抵抗细胞模型，予以不同浓度4-HIL（5、10、20 μmol/L）进行干预。采用［3H］－脱氧-D-葡萄糖摄入法检测葡萄糖摄取率，PCR法检测肿瘤坏死因子-α（tumor necrosis factor-alpha，TNF-α）mRNA表达量，ELISA法检测细胞上清TNF-α蛋白水平。以棕榈酸（palmitic acid，PA）为对照。结果 25 mmol/L葡萄糖加0.6 nmol/L胰岛素作用18 h后，3T3-L1脂肪细胞胰岛素刺激的葡萄糖转运抑制了63％；PCR显示脂肪细胞内TNF-α mRNA的表达较正常脂肪细胞明显增高（P<0.05）；ELISA显示细胞上清中的TNF-α分泌量增加70 pg/mL（P<0.05）。PA组表现出类似结果。分别加入5、10、20 μmol/L 4-HIL作用24 h后，3T3-L1脂肪细胞胰岛素刺激所产生的葡萄糖转运分别增加35％、50％、60％；PCR检测显示，脂肪细胞内TNF-α mRNA的表达随着药物浓度的增加呈递减趋势，与模型组及PA组比较，差异有统计学意义（P<0.05）；与模型组比较，药物干预后脂肪细胞培养基上清TNF-α分泌水平分别下降10、18、39 pg/mL（P<0.05）。结论 4-HIL可以显著改善高糖诱导的小鼠3T3-L1脂肪细胞胰岛素抵抗，同时4-HIL能够抑制TNF-α的分泌。 |
| 英文摘要: |
| ObjectiveTo investigate the effect of 4-hydroxyisoleucine （4-HIL）， an active component of Trigonella Foenum-graecum L. on high glucose induced insulin resistance （IR） in 3T3-L1 adipocytes， and to explore underlying molecular mechanisms. Methods3T3-L1 adipocytes were treated with 25 mmol/L glucose and 0.6 nmol/L insulin to induce IR. They were intervened by different concentrations of 4-HIL （at 5， 10， and 20 μmol/L）. ［3H］-Deoxy-D-glucose up-taking method was used to detect the glucose uptake. The mRNA expression of cellular tumor necrosis factor-alpha （TNF-α） was detected by polymerase chain reaction （PCR）. The content of TNF-α in the culture supernatant was detected by enzyme-linked immunosorbent assay （ELISA）. Palmitic acid （PA） acted as the control. ResultsAfter intervened by 25 mmol/L glucose and 0.6 nmol/L insulin for 18 h， the insulin-stimulated glucose transportation in 3T3-L1 adipocytes was inhibited by 63%. The mRNA expression of cellular TNF-α in adipocytes significantly increased， when compared with that in normal adipocytes （P<0.05）. The level of TNF-α secreted in the culture supernatant was increased by 70 pg/mL （P<0.05）. Similar changes occurred in the PA group. After exposure to 4-HIL （5， 10， or 20 μmol/L） for 24 h， the glucose transportation was increased by 35%， 50%， and 60%， respectively. PCR results showed that along with increasing 4-HIL concentrations， the mRNA expression of cellular TNF-α showed a decreasing trend， showing statistical difference when compared with the model group and the PA group （P<0.05）. Compared with the model group， the TNF-α level in the supernatant was respectively reduced by 10 pg/mL， 18 pg/mL， and 39 pg/mL after intervention （P<0.05）. Conclusion4-HIL could remarkably improve high glucose-induced IR in 3T3-L1 adipocytes. Meanwhile， 4-HIL could inhibit the secretion of TNF-α. |
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