| 王淑慧;张丽;郝延芝;蒋敏;吕竞芳;张玉倩;于志芳;刘孟瑞;杜惠兰.寿胎丸对复发性流产小鼠蜕膜组织有氧糖酵解抑制的影响[J].,2024,44(1):56-62 |
| 寿胎丸对复发性流产小鼠蜕膜组织有氧糖酵解抑制的影响 |
| Effect of Shoutai Pill on Aerobic Glycolysis Inhibition in Decidual Tissue of Recurrent Spontaneous Abortion Mice |
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| DOI：10.7661/j.cjim.20230726.281 |
| 中文关键词: 寿胎丸 有氧糖酵解 蜕膜 复发性流产 乳酸 凋亡 中药复方 |
| 英文关键词:Shoutai Pill aerobic glycolysis decidua recurrent spontaneous abortion lactic acid apoptosis Chinese herbal compound |
| 基金项目:国家自然科学基金联合资助项目（No.U21A20403） |
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| 中文摘要: |
| 目的 观察寿胎丸防治复发性流产（RSA）的作用，探讨其调控有氧糖酵解的作用。方法 将40只CBA/J小鼠随机分为正常组、RSA组、地屈孕酮组和寿胎丸组，每组10只，分别灌胃蒸馏水、地屈孕酮溶解剂、寿胎丸14天。14天后将正常组CBA/J雌鼠与BALB/c雄鼠2∶1合笼，其余3组雌鼠与DBA/2雄鼠2∶1合笼，建立RSA模型，并按照前法继续灌胃至见栓第10天。计算各组小鼠胚胎丢失率，HE染色观察蜕膜组织，Western Blot、实时荧光定量PCR技术（qRT-PCR）检测小鼠蜕膜组织己糖激酶（HK）2、M2型丙酮酸激酶（PKM2）、乳酸脱氢酶（LDH）A、B淋巴细胞瘤-2（Bcl-2）、B细胞淋巴瘤-2相关X蛋白（Bax）、半胱氨酸天冬氨酸蛋白酶3（Caspase-3）表达，乳酸脱氢酶比色法检测乳酸（LA）含量，检测HK、PK、LDH活性。结果 与正常组比较，RSA组小鼠胚胎丢失率升高（P＜0.05），蜕膜组织细胞减少，排列疏松，胞浆空泡样变，部分细胞核消失，蜕膜组织HK2、PKM2、LDHA、Bcl-2蛋白及mRNA表达降低（P＜0.05），Bax、Caspase-3蛋白及mRNA表达增高（P＜0.05）；HK、PK、LDH活性降低（P＜0.05），LA含量降低（P＜0.01）。与RSA组比较，地屈孕酮组、寿胎丸组小鼠胚胎丢失率降低（P＜0.05），蜕膜组织细胞排列较致密，少数蜕膜细胞胞浆水肿、空泡样变，胞质较丰富，小鼠蜕膜组织HK2、PKM2、LDHA、Bcl-2蛋白及mRNA表达升高（P＜0.05），Bax、Caspase-3蛋白及mRNA表达降低（P＜0.01）；HK、PK、LDH活性升高（P＜0.05），LA含量升高（P＜0.01）。结论 寿胎丸可通过调节蜕膜组织有氧糖酵解水平，提高LA含量，减少蜕膜细胞凋亡，从而降低胚胎丢失率，起到防治RSA的作用。 |
| 英文摘要: |
| Objective To observe the effect of Shoutai Pill on the prevention and treatment of recurrent spontaneous abortion （RSA）， and to study its effects of regulating aerobic glycolysis. Methods Forty CBA/J
mice were randomly divided into normal group， RSA group， dydrogesterone group， and Shoutai Pill group， 10 mice in each group. The mice were administered with distilled water， dydrogesterone solubilizer， and Shoutai Pill by gastrogavage for 14 successive days， respectively. After 14 days， female CBA/J rats in normal group and male BALB/c rats in BALB/ c group were mated at 2：1， and female rats in other 3 groups were mated with male DBA/2 rats at 2：1. RSA model was established， and gastrogavage was continued until the 10th day of embolization according to previous methods. The rate of mouse embryo loss in each group was calculated. The decidua tissue was observed by HE staining. The expressions of hexokinase （HK） 2， M2 pyruvate kinase （PKM2）， lactate dehydrogenase （LDH）A， B-cell lymphoma-2 （Bcl-2）， B-cell lymphoma-2 associated X protein （Bax）， and cysteinyl aspartate specific protease-3 （Caspase-3） in the decidua tissue of mice were detected by Western Blot and Real-time fluorescence quantitative PCR （qRT-PCR）. Lactic acid（LA） content was detected by lactate dehydrogenase colorimetry. The activities of HK， PK， and LDH were also detected. Results Compared with the normal group， the embryo loss rate of mice in RSA group increased （P＜0.05）， decidua tissue cells were reduced with loose arrangement， cytoplasm vacuolated， and partial nuclei disappeared. And the protein and mRNA expressions of HK2， PKM2， LDHA， and Bcl-2 decreased （P＜0.05）. The protein and mRNA expressions of Bax and Caspase-3 increased （P＜0.05）. And the activities of HK， PK， and LDH decreased （P＜0.05）， and LA content lowered （P＜0.01）. Compared with RSA group， the embryo loss rate of mice in dydrogesterone group and Shoutai Pill group decreased （P＜0.05）. The arrangement of decidual tissue cells was relatively dense， with cytoplasmic edema in few decidual cells， cytoplasm vacuolated， and richer cytoplasm. And the protein and mRNA expressions of HK2， PKM2， LDHA， and Bcl-2 in the decidual tissue of mice increased （P＜0.05）. The protein and mRNA expressions of Bax and Caspase-3 decreased （P＜0.01）. The activities of HK， PK， and LDH increased （P＜0.05）， and LA content increased （P＜0.01）. Conclusion Shoutai Pill played roles in preventing and treating RSA possibly through regulating the level of aerobic glycolysis in decidua tissue， elevating the content of lactic acid， reducing the apoptosis of decidua cells， and further reducing the embryo loss rate. |
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