利用实验动物模型研究中草药内用治疗银屑病的作用

作者利用小鼠阴道上皮和鼠尾鳞片表皮两个实验动物模型,研究了三种临床上报告内用治疗银屑病有效的中草药有效成份的作用机理,见到喜树碱能显著地抑制上皮细胞有丝分裂并促进表皮颗粒层形成,肿节风浸膏主要是促进表皮颗粒层形成,而苦参素则只有抑制上皮细胞有丝分裂的作用。利用这两个实验模型对喜树碱、肿节风浸膏和苦参素的疗效及毒性,以氨甲喋呤为对照进行评价,结果与临床观察大致相符。

英文摘要:

Using mouse vaginal epithelium and mouse tail-scale epidermis as experimental animal models,the authors studied the therapeutic mechanism of 3 kinds of Chinese herbal drugs reported to be clinically effective in systemic therapy of psoriasis.It was observed that intraperitoneal injection of camptothecine significantly reduced the mitosis of mouse vaginal epithelium and promoted the formation of a granular layer in the mouse tail-scale epidermis,suggesting that the therapeutic mechansim of the drugs may be on the one hand suppressing-hyperplasia of the psoriatic epidermis,and,on the other,turning parakeratosis into ortho- keratinization.The main effect of Sarcandra glaber N.extract is to promote the formation of a granular layer.Oxymatrine-oxysophocarpine mixture can only reduce the mitosis of epithelial cells.Methotrexate,used as a positive control,reduces the mitosis of epithelial cells in a degree similar to camptothecine,but its promotive effect on the formation of a granular layer is stronger than camptothecine. Considering that decrease of mitosis not only accounts for the therapeutic effect of a drug,hut also represents its toxicity,an experimental assessment on the therapeutic and toxic effects of the above-men- tioned drugs can be made as the following:(1)methotrexate possesses a significant therapeutic effect,while its toxicity is obvious;(2)camptothecine is a little less effective than methotrexate,but it still shows a significant toxicity;(3)Sarcandra glaber N.extract is less effective than camptothecine,but it shows no obvious toxicity; (4)Oxymatrine-oxysophocarpine mixture is also less effective than camptothecine,and its therapeutic effect may be associated with its toxicity.This experimental assessment appears to be considerably correspondent with the reported clinical observations.

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