缺血心肌中环核苷酸的改变及槲寄生对其影响

作者	单位
朱顺和	上海市第二医学院附属第三人民医院心血管研究室
俞国瑞	上海市第二医学院附属第三人民医院心血管研究室
黄铭新	上海市第二医学院附属第三人民医院心血管研究室
成柏华	上海第二医学院针麻研究室
郑道声	上海市第二医学院附属第三人民医院心血管研究室
陈曙霞	上海市第二医学院附属第三人民医院心血管研究室

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中文摘要:

本实验结扎大白鼠左前降支冠状动脉,造成心肌缺血区域,测定缺血和非缺血心肌内 cAMP 和 cGMP 的含量,算得 A/G 的比值,显示心肌缺血1小时时比对照区域心肌和正常心肌的 cAMP 含量、A/G 比值增高,结扎前腹腔内注射槲寄生液者,缺血心肌中 cAMP 含量显著降低,A/G 比值亦降低。心肌 cAMP 含量增高与心律失常和心肌缺血的严重程度密切有关,本文提出槲寄生是一有希望的保护心肌的中药。

英文摘要:

The increase of cAMP content in ischemic myocardium is one of the major biochemical mechanisms that make ischemic injury worse and cause arrhythmia.It is possible to treat arrhythmia and keep ischemic myocardium under control by decreasing its level in ischemic myocardium or disrupting its effects.In this experiment,we ligated the proximal end of the left anterior descending coronary artery of mice.The cAMP content in myocardium infarcted for 1 hour(9.95 pmoles/mg wet weight,M±SE)was significantly higher then that of sham operated myocardium(1.78±0.70)and that of non-infarcted myocardium(0.86±0.10, P<0.05).At the same time,cGMP level of ischemic myocardial tended to go higher than that of the sham operated(0.14±0.045 vs 0.04±0.018),(0.05<P<0.1).As the cAMP/cGMP ratio of the ischemie is significantly higher than that of the non-infarcted(125.8±27.8 vs 28.2±1.59,P<0.05)and that of the sham operated(41.3±26.9),(0.05<P<0.1),excessive sympathetic activity in the ischemic myocardium is thus indicated.Viscum coloratum(kom)Nakai injectiones were then injected into the peritoneal cavities of the mice(80 μg/g body weight)before ligation,the increase of cAMP in the ischemic myocardium group was shown to be significantly suppressed(0.94±0.26),and the cAMP/cGMP ratio decreased(53.1±16.3), (0.05<P<0.1).These results suggest that Viscum coloratura(kom)Nakai may counteract β-receptor directly or alleviate the sympathetic reflexes,and that it may be a promising cardioprotective Chinese traditional drug.

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