苦参碱抗炎症作用的实验研究

将苦参碱给小鼠肌肉注射,其 LD₅₀为74.15±6.14mg/kg。苦参碱与氢化可的松相似,肌肉注射都能明显对抗巴豆油诱发小鼠和大鼠耳壳的炎症,长期给药时其作用随剂量增加而增强。它对大鼠后肢由角叉菜胶诱发的炎症和对小鼠腹腔注射冰醋酸诱发的渗出性炎症均有明显的抑制作用;但对大鼠由埋藏棉球诱发肉芽组织增生的慢性炎症却无影响,而氢化可的松则有明显的对抗作用。在分析苦参碱的抗炎症作用机理时发现,它对垂体—肾上腺系统无明显影响。管内实验发现苦参碱能明显对抗血清蛋白加热变性的作用;对红细胞的溶血现象也有抑制作用。

英文摘要:

Matrine（MT）is a kind of alkaloid,extracted from Sophora Alopeeuroides L.which contains about 0.06%.The LD₅₀of MT of mice caused by intramuscular injection was found to be 74.15±6.14 mg/kg. Administration of MT（15 or 25 mg/kg,im in mice;25 mg/kg im in rats）decreased markedly the inducement of ear inflammation in mice or rats by the croton oil,and the K-Carrageenin-induced hind paw edema in rats.After intramuscular injection of MT（15 and 25 mg/kg per day）and hydrocortisone（150mg/kg per day）for 8 days in rats, the inducement of ear inflammation in rats by the croton oil was decreased with an anti-inflammatory rate of 31.74% （P<0.01）,51.89%（P<0.005）and 76.06%（P<0.001）respectively.30 minutes after intramuscular injection of MT（9 and 15 mg/kg）and hydrocortisone（200 mg/kg）in mice,the inflammation by acetic acid（ip）shown by the infiltration of Even’s blue decreased singnificantly,and the anti-inflammatory rates were 11.0%（P>0.05）,25.7%（P<0.05）and 67.4%（P<0.001）respectively. In mice,extirpation of adrenal glands did not influence the anti-inflammatory effect of MT.It is suggested that its effect is not related to the hypothalamie adrenal system.Protein denaturation and red blood cells hemolysis with heat in vitro could be inhibited by MT.In chronic inflammation experiments,MT failed to inhibit the proliferation of granuloma induced by the implantation of cotton pellets in rats.The result suggests that MT exhibits the characteristics of nonsteroid anti-inflammatory agents.

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