防感合剂抗病毒及诱生干扰素的作用观察

本文采用空斑法、微量细胞病变抑制法、以及对小鼠进行鼻腔攻击的方法,观察了防感合剂的抗病毒及诱生干扰素作用。结果表明,防感合剂可直接灭活呼吸道合胞病毒(RSV)和对 RSV 感染后有保护作用,并可降低小鼠在副流感痛毒攻击下的死亡率。此外,防惑合剂还有对新城鸡瘟病毒(NDV)诱生干扰素的协同增强作用。在小鼠腹腔注射防感合剂后的8、16、24、32h,用微量细胞病变抑制法(CPE)在 L929细胞上测定干扰素效价,其中24h 数值最高为98.23u/ml。所诱生的干扰素符合Ⅰ型干扰素性质。

英文摘要:

The present study was carried out by using the plaque formation test,microdosage cytopathic effect（CPE）method,and mice nasal-cavity-attacking method to observe the antiviral and interferon-inducing effect of Fang-Gan mixture（FGM）.The results showed that FGM has direct respiratory syncytia virus（RSV）inactivation effect,and protection effect after mice infected by RSV.FGM could also decrease mice death rate（P<0.01）in the experimentation of using parainfluen- za virs to attack them,and work in coordination with interferon induced by Newcastle disease virus.After 8,16,24,32 hours of injecting FGM into mice abdominal cavity,the authors used CPE method to determine interferon titer of L₉₂₉ cells,and found that the highest interferon titer was 98. 23 u/m at the 24th hour.The interferon induced by FGM was in keeping with the nature of Type Ⅰ interferon.The results suggested that FGM might have strong antiviral effect which is achieved by inducing interferon.

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