四君子汤对小鼠巨噬细胞细胞毒功能的影响

采用⁵¹Cr,¹²⁵I-UdR 释放法,检测四君子汤对正常和免疫抑制小鼠腹腔巨噬细胞介导的肿瘤细胞溶解作用和抗体依赖细胞介导的细胞毒作用的影响。结果发现:四君子汤0.4ml/20mg 每天1次连续灌胃7天,对正常小鼠腹腔巨噬细胞介导的细胞毒活性并无明显提高。给小鼠腹腔单独注射环磷酰胺（Cy,100mg/kg）后,腹腔巨噬细胞介导的细胞毒活性均显著降低。当加用四君子汤处理小鼠后,上述两种细胞毒活性比单用 Cy 组明显提高,增强率分别为74.40%和121.03%。表明四君子汤能拮抗 Cy 对小鼠腹腔巨噬细胞介导的细胞毒活性的抑制作用,而对正常小鼠影响不明显。提示四君子汤对小鼠的免疫调理作用与机体的免疫状态有关。

英文摘要:

The effect of Si Jun Zi Tang（SJZT）on the activities of peritoneal macrophage（Mφ）mediated MTC and ADCC in normal and immunosuppressed mice were examined by isotope releasing assay (⁵¹Cr,¹²⁵I-UdR).The results showed the SJZT no significantly increased peritoneal Mφ.mediated cytotoxic activity（MTC,ADCC）in normal mice.After the injection of immuno-depressive cyclo- phosphamide（Cy）alone（ip）,the cytotoxic activities were decreased,but by treatment with Cy and SJZT,the cytotoxic activities were significartly increased compared with Cy group,the enhancing rates amounted to 74.40%（Mφ.MTC）and 121.03%（Mφ.ADCC）respectively.It was demonstrated that the SJZT could protest against the effect that the Cy inhibited the activities of MTC and ADCC of peritoneal Mφ in mice,but there was no significant effect on normal mice.It indicated that the function of SJZT’s immunoregulation was related to the state of immune in body.

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