生血灵对原发性血小板减少性紫癜患者骨髓体外培养巨核系祖细胞的影响

作者	单位
周永明	上海中医学院附属岳阳医院血液科上海200031
薛志忠	上海中医学院附属岳阳医院血液科上海200031
姚楚芳	上海中医学院附属岳阳医院血液科上海200031
张艇	上海中医学院附属岳阳医院血液科上海200031
张琴明	上海中医学院附属岳阳医院血液科上海200031
黄振翘	上海中医学院附属岳阳医院血液科上海200031
陆敬懿	上海中医学院附属岳阳医院血液科上海200031

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中文摘要:

采用改良的甲基纤维素-琼脂双层体外培养技术,观察生血灵对原发性血小板减少性紫癜(ITP)患者骨髓巨核系祖细胞(CFU-Meg)的影响。结果表明:ITP 患者 CFU-Meg 平均产率明显低于正常对照组(P<0.01);加入生血灵200mg/ml 时平均产率显著增加(P<0.05);加入300mg/ml 和500mg/ml 时增加均非常显著(P<0.01);而正常骨髓细胞培养体系同时加入 ITP 患者血清和生血灵培养后,平均产率显著高于患者组(P<0.01)且接近正常对照组(P>0.05)。提示生血灵能抑制抗血小板抗体,促进 ITP 患者 CFU-Meg的增殖、分化和成熟。

英文摘要:

This paper used an improved methyllulose-agar double layer method in vitro to culture megakaryocyte progenitors(CFU-meg)of 15 normal bone marrow donors and 20 idiopathic throm- botytopenic purpura(ITP)patients bone marrow cells in order to observe the action of Shen-Xue- Ling.(SXL).The results showed that the colony efficiency of CFU-meg of ITP group(13.75±6.93/10~5 MNC)was obviously lower than that of the control group(21.22±9.06/10~5 MNC,P<0.01).The serum of the patients whose PAIgG was markedly higher than normal value inhibited growth of CFU-meg derived from normal bone marrow cells(P<0.01).SXL could increase the colony efficiency of CFU-meg for ITP patients at several doses.The average colonies were 15.77±6.31/10~5 MNC(at 100 mg/ml,P>0.05),18.72±5.54/10~5MNC(at 200 mg/ml,P<0.05),21.60±7.35/10~5 MNC(at 300 mg/ml, P<0.01)and 21.84±7.15/10~5 MNC(at 500 mg/ml,P<0.01)as compared with the group of ITP patients.Incubated with patient’s serum(1%)and SXL(at 300 mg/ml),the number of CFU-meg extracted from normal bone marrow cells.was significantly higher than that from patients(P<0.01) and was close to normal group(P>0.05).The results suggested that SXL might inhibit the anti- platelet antibodies,facilitate reproduction,division and maturity of CFU-meg.

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