寒体与热体的实验研究(Ⅱ)——寒体与热体大鼠淋巴细胞体外增殖及DNA损伤后复制能力的比较观察

作者	单位
丁镛发	上海中医学院上海 200032
钱汝红	上海中医学院上海 200032
匡调元	上海中医学院上海 200032
张伟荣	上海中医学院上海 200032
沈雁	上海中医学院上海 200032
李伯勤	上海中医学院上海 200032
应馨萍	上海中医学院上海 200032
肖颐	上海中医学院上海 200032
张俊	上海中医学院上海 200032

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中文摘要:

从 Wistar 大鼠的自然群体中筛选出热体、寒体与常体大鼠,作为研究对象。用动物的外周血和脾脏作材料,通过细胞体外培养、紫外线损伤、同位素掺入及液闪测定等方法,观察比较各组大鼠脾淋巴细胞的体外增殖能力,外周淋巴细胞 DNA 损伤后的复制合成能力。结果表明,在此两项实验指标中,热体大鼠比寒体大鼠具有更高的水平,而常体大鼠居中。因而从一个方面证实了《灵枢》所称"其身多热者易已;多寒者,难已"的科学性。

英文摘要:

The cold-constitutional and the heat-constitutional type had selected in Wistar rats asthe object of study.Using peripheral blood and spleen as materials by means of ultraviolet injury,isotope incorporation,cells incubation in vitro and liquid scintillation counting,the capacity of peripheral lymphocytes DNA replication after damage with ultraviolet radiation and the capacity of the spleen lymphocytes proliferation in vitro was observed. The results showed that the both capacities mentioned above were higher in the heat constitutional type rat than that in the cold type.It is suggested that the following conclusion in the Lingshu Jing(灵枢经)is correct:"the capacity of tissue repair is higher in the heat constitutional type than in the cold."

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