二仙汤及其拆方对老年大鼠部分抗氧化酶活性及其基因表达水平的影响

作者	单位
沈小瑜	上海第二医科大学附属瑞金医院
方肇勤	上海中医药大学
吴敦序	上海中医药大学
顾文聪	上海中医药大学
宋菊敏	上海中医药大学
廖菡	上海中医药大学
张伯讷	上海中医药大学

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中文摘要:

采用分子生物学实验技术对二仙汤及其拆方影响老年大鼠肝组织超氧化物歧化酶（ＳＯＤ），过氧化氢酶（ＣＡＴ）活性及其基因表达水平的作用进行了观察。结果显示：老龄大鼠过氧化脂质（ＬＰＯ）含量明显增高；ＳＯＤ、ＣＡＴ，活性降低；而且酶活性降低与这类酶蛋白的基因（ｍＲＮＡ）表达水平减弱密切相关。用二仙汤及其拆方治疗后，能明显降低老龄大鼠肝组织ＬＰＯ含量；提高ＳＯＤ和ＣＡＴ活性；并观察到这些酶活性的提高又与编码相应酶蛋白的ｍＲＮＡ表达水平增强有关。提示二仙汤可在基因水平上，通过调控、增强编码酶蛋白的基因表达水平而增强抗氧化酶活性，减少自由基产物的作用，从而具有延缓衰老的疗效。

英文摘要:

In order to study the mechanism of aging,this experiment adopted molecular biological techniques to observe the effect of Er-Xian decoction(EXD) and its disassembled prescription on the enzyme activities and their gene expression of supoxide dismutase(SOD)and catalase(CAT).The results showed that in old rat,the amount of lipid peroxide(LPO) increased significantly;the enzyme activities such as SOD,CAT decreased significantly;and the lowering of these activities had a direct bearing on the gene expression level of their enzyme protein.After treatment by EXD and its disassembled prescription in aging rat,the amounts of LPO reduced significantly,the activities of SOD,CAT enhanced significantly.Moreover,it was observed that the increasing of these enzyme activities was related to the enhancing of their gene expression. It was suggested that EXD had the effect of anti-aging in that it could raise the activities of antioxidant enzymes and reduce the product of free radicals by means of regulating and enhancing the gene expression levels of their enzyme protein.

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