补肾健脾活血三类复方对下丘脑-垂体-肾上腺-胸腺轴及CRF基因表达的影响

Effect of Three Kinds(Tonifying Kidney, Invigorating Spleen, Promoting Blood Circulation) Recipes on the Hypothalamus-Pituitary-Adrenal-Thymus(HPAT)Axis and CRF Gene EXpression

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DOI：

中文关键词: 补肾健脾活血促肾上腺皮质激素释放因子信使核糖核酸下丘脑皮质酮

英文关键词:tonifying the Kidney invigorating the Spleen promoting blood circulation Corticotropin-releasing factor messenger ribonucleic acid hypothalamus corticosterone

基金项目:国家自然科学基金

作者	单位
钟历勇
沈自尹
蔡定芳
郑仲承
陈晓红

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中文摘要:

目的：为从分子水平来阐明药物对肾阳虚证的主要调节点。方法：采用逆转录多聚酶链反应（ＲＴ－ＰＣＲ）化学发光定量法、放射免疫及细胞免疫技术，观察补肾、健脾、活血三类复方分别对下丘脑一垂体一肾上腺一胸腺（ＨＰＡＴ）轴受抑制模型的下丘脑促肾上腺皮质激素释放因子（ＣＲＦ）ｍＲＮＡ表达、神经内分泌和免疫功能的影响。结果：唯有补肾药可通过提高下丘脑ＣＲＦｍＲＮＡ表达来保护ＨＰＡＴ轴免受外源性皮质酮的抑制；健脾药对免疫系统有直接的促进作用；而活血药对ＨＰＡＴ轴无任何影响。结论：药物对肾阳虚证的主要调节点定位在下丘脑。

英文摘要:

To observe the effect of tonifying Kidney (TK), invigorating Spleen (IS) and promoting blood circulation (PBC) recipes on the hypothalamus-pituitary -adrenal (HPAT)axis. Methods: reverse-transcription polymerase chain reaction, radioimmunoassay and cellular immunity techniques were used to observe the effects of TH, IS, PBC on the neuroendocrine and immune system of this experimental model which HPAT axis was inhibited by corticosterone.Results: only TK could avoid the depression of exogenous corticosterone, which by enhancing the expression of CRFmRNA in hypothalamus, and following the improvement of function of HPAT axis, that IS might have direct promotion on immune system, whereas without any effect of PBC on HPAT axis was observed. Conclusion: TK, IS and PBC recipes have different effects on HPAT axis.

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