川芎嗪对免疫介导再生障碍性贫血小鼠骨髓细胞CD<sub>34</sub>抗原表达的影响

作者	单位
舒砚君	同济医科大学附属同济医院!武汉430030
孙汉英	同济医科大学附属同济医院!武汉430030
董凌莉	同济医科大学附属同济医院!武汉430030
徐慧珍	同济医科大学附属同济医院!武汉430030
路武	同济医科大学附属同济医院!武汉430030
刘文励	同济医科大学附属同济医院!武汉430030

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中文摘要:

探讨川芎嗪对免疫再生障碍性贫血（再障）小鼠骨髓细胞ＣＤ_３４抗原分子表达的影响。方法：建立免疫介导的再障小鼠模型，６．０Ｇｙ～^６０Ｃｏγ－射线照射和尾静脉输注淋巴细胞造成小鼠再障。分为正常组、再障组（对照组）、川芎嗪组，除正常组外均胃饲川芎嗪注射液４ｍｇ／次，１天２次，第１０天用流式细胞仪检测各组小鼠骨髓细胞膜上ＣＤ_３４抗原表达量。结果：川芎嗪ＣＤ_３４抗原表达量的荧光强度（７７．６±６．５）明显高于再障组（６８．６±４．５，Ｐ＜０．０５），与正常组（８０．０±２．６）比较无明显差异。结论：川芎嗪通过影响骨髓微环境促进免疫再障小鼠的造血干、祖细胞增生，增加ＣＤ_３４抗原分子的表达。

英文摘要:

To explore the effect of ligustrazine on CD₃₄ antigen expression of bone marrow cells in imrnunemediated aplastic anemia （AA）mice. Methods: The model of immune aplastic anemia mice was induced by means of 6.0Gy⁶⁰Co γ-ray irradiation and lymphocyte infusion through tail vein. The mice were divided into 3 groups: the normal group, the AA control group and the ligustrazine group. Mice of the ligustrazine group were fed by 4 mg of ligustrazine injection twice a day by gastrogavage. On the 10th day, CD₃₄ antigen expression intensity of bone marrow cell membrane was measured by flow cytometer analysis system. Results: CD₃₄ antigen expression intensity of ligustrazine group was 77.6±6. 5, with no statistic difference from that in the normal group （80.0±2. 6）, while that of the control group was much higher （68.6±4.5, P<0.05）. Conclusions: Ligustrazine could promote proliferation of stem and progenitor cell of AA mice through influencing on bone marrow micro-environment so as to increase the CD₃₄ antigen expression of bone marrow cells.

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