不同补肾方剂对老年前期小鼠不同脏器细胞核体外RNA转录和无细胞提取液蛋白质合成活性的影响

Effect of Different Prescriptions for Tonifying Kidney on RNA Transcription Activity in Isolated Nuclei and Protein Synthesis Activity in Free Cell Extracts of the Different Organs of Presenile Mice

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中文关键词: 左归饮益肾宝老年前期 RNA转录蛋白质合成

英文关键词:Zuoguiyin Yishenbao presenile mice RNA transcription protein synthesis

基金项目:国家中医药管理局科研基金资助课题 (No.92 2 2 0 3)

作者	单位
张杰	中国中医研究院基础理论研究所!北京100700
崔成德	中国中医研究院基础理论研究所!北京100700
徐琦	中国中医研究院基础理论研究所!北京100700

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中文摘要:

目的 :探讨补肾阴方左归饮和补肾阳方益肾宝对老年前期小鼠抗衰老的机制。方法 :用 1 4月龄雄性小白鼠为肾虚模型 ,观察左归饮及益肾宝对其肝、脑、肾、睾丸的细胞核体外RNA转录活性及无细胞提取液蛋白质合成活性的影响。结果 :老年前期对照组小鼠 4种脏器的细胞核体外RNA转录活性及无细胞提取液蛋白质合成活性明显低于青年组 (3月龄 )小鼠。经灌服左归饮和益肾宝 2个月后 ,两给药组小鼠 4种脏器的细胞核体外RNA转录活性及无细胞提取液蛋白质合成活性均明显高于老年前期对照组小鼠 ,而两给药组间无明显差异。结论 :补肾阴与补肾阳方剂均可提高老年前期肾虚小鼠的RNA转录活性及蛋白质合成活性

英文摘要:

Objective: To study the anti aging mechanism of Zuoguiyin (a prescription for tonifying Kindney Yin) and Yishenbao (a prescription for tonifying Kidney Yang) in presenile mice. Methods: Using male, 14 months old presenile mice as a model of Kidney Asthenia, the effects of Zuoguiyin and Yishenhao on RNA transcription activity in isolated nuclei and protein synthesis activity in free cell extracts of the liver, brain, kidney and testes of the presenile mice were observed. Results: RNA transcription activity in isolated nuclei and protein synthesis activity in free cell extracts of the four organs of the presenile mice group were distinctly lower than those of young mice group (3 months old). After above mentioned two prescriptions were given to the presenile mice for two months, RNA transcription activity in isolated nuclei and protein synthesis activity in free cell extracts of the four organs of the two treated groups were obviously higher than those of control group (P<0 05 or P<0 01),but there was no difference between two treated groups. Conclusions: Both Zuoguiyin and Yishenbao could promote RNA transcription activity in isolated nuclei and protein synthesis activity in free cell extracts of Kidney Asthenia and presenile mice.

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