515抗瘤方剂诱导白血病HL60细胞凋亡的研究

作者	单位
谢新生	山东省立医院!济南250021
高聆	山东省立医院!济南250021
宋玉琴	山东省立医院!济南250021
王春霞	山东省立医院!济南250021
赵家军	山东省立医院!济南250021
徐功立	山东省立医院!济南250021

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中文摘要:

目的:观察中药515抗瘤方剂对白血病HL60细胞的影响。方法:采用光镜、透射电镜观察形态及结构,流式细胞术测量凋亡率,原位末端标记检测DNA断裂。结果:细胞与药物共同孵育一定时间后出现核固缩、核碎裂等典型的凋亡形态学变化,药物浓度在0013～004g/ml之间,药物组与对照组凋亡率有显著性差异(P<001)。结论:515抗瘤方剂能诱导HL60细胞发生凋亡,细胞凋亡率随时间延长增加,且呈现剂量依赖性。

英文摘要:

To observe the efficacy of 515 anti tumor recipe on inducing human myeloid leukemic HL 60 cells apoptosis Methods: The morphological change of apoptotic cells was observed by light microscopy and transmission electromicroscopy (TEM), and flow cytometry and TUNEL reaction to further confirm the apoptosis of HL 60 cells. Results: Typical apoptosis appeared such as marginal nuclei, chromatin condensation and nuclear fragmentation could be seen by light microscopy and TEM. The drug concentration was between 0.013 ～0.04g/ml. The apoptosis rate is 30% in 0.04g/ml group at the 72nd hour, but it was only 0.8% in the control group at the same time (P<0 01). Conclusions: The 515 anti tumor recipe can induce apoptosis in HL 60 cells. The apoptosis rate increased as the time extended. There was an enhanced efficiency of apoptosis in a dose dependent manner.

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