中华眼镜蛇蛇毒组分C诱导白血病细胞凋亡作用的研究

作者	单位
郝玉书	TAN Huo, HAO Yushu, YING Hongguang, et al (The Second Affiliated Hospital of Guangzhou Medical College, Guangzhou (510260
应红光	TAN Huo, HAO Yushu, YING Hongguang, et al (The Second Affiliated Hospital of Guangzhou Medical College, Guangzhou (510260
林振桃	TAN Huo, HAO Yushu, YING Hongguang, et al (The Second Affiliated Hospital of Guangzhou Medical College, Guangzhou (510260
管锦霞	TAN Huo, HAO Yushu, YING Hongguang, et al (The Second Affiliated Hospital of Guangzhou Medical College, Guangzhou (510260
张广森	TAN Huo, HAO Yushu, YING Hongguang, et al (The Second Affiliated Hospital of Guangzhou Medical College, Guangzhou (510260

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中文摘要:

研究眼镜蛇蛇毒组分Ｃ诱导白血病细胞凋亡的作用及机制。方法：应用光学显微镜、透射电镜、脱氧核糖核酸（ＤＮＡ）电泳、流式细胞仪、逆转录聚合酶链反应（ＲＴ－ＰＣＲ）等方法，观察白血病细胞经过眼镜蛇蛇毒组分Ｃ处理后，形态学、生物化学及ＢｃＩ－２／Ｂａｘ基因表达水平的变化。结果：眼镜蛇蛇毒组分Ｃ能诱导人粒细胞白血病细胞系（ＨＬ６０）发生凋亡形态学和生物化学变化；流式细胞议分析发现眼镜蛇蛇毒组分Ｃ能使一定比例的人粒单细胞白血病细胞系（Ｊ６－１）、人红白血病细胞系（Ｋ５６２）、ＨＬ６０。细胞及取自患者骨髓的白血病细胞发生凋亡，而且凋亡率随蛇毒浓度的增高而增加；ＲＴ－ＰＣＲ方法检测发现眼镜蛇蛇毒组分Ｃ能使ＨＬ６０细胞Ｂｃｌ－２基因的表达下调，而Ｂａｘ变化不明显。结论：眼镜蛇蛇毒组分Ｃ能诱导白血病细胞发生凋亡，此作用与Ｂｃｌ－２基因的表达下调有关。

英文摘要:

To study the effect and mechanism of fraction C isolated from Naja naja Actra Venom (FCNNAV) in inducing apoptosis of human leukemia cells. Methods: Light microscope, transmission electron microscope, DNA electrophoresis, flow cytometry and RT-PCR assay were used to observe the changes of human leukemia cells in morphology and biochemistry, and Bcl-2/Bax expression after exposing to FCNNAV. Results: FCNNAV could induce HL60 cells apoptosis demonstrated by the typical morphological and biochemical changes. Flow cytometry showed that J6-1, K562, HL60 and fresh leukemia cells apoptosis could be induced by FCNNAV, and the apoptosis rate was dose-dependent. RT-PCR detection showed the Bcl-2 gene expression of HL60 cells was down-regulated by FCNNAV, whereas the Bax expression was unaffected. Conclusion: FCNNAV could induce apoptosis of human leukemia cells and this effect is related to down-regulation of Bcl-2 gene expression level.

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