补肾清利活血法对大鼠残肾细胞外基质影响的实验研究

作者	单位
阳晓	YANG Xiao, WEI Yi, LIU Xiaowen, et al. Hunan Medical College of TCM, Changsha (410007
魏毅	YANG Xiao, WEI Yi, LIU Xiaowen, et al. Hunan Medical College of TCM, Changsha (410007
刘晓文	YANG Xiao, WEI Yi, LIU Xiaowen, et al. Hunan Medical College of TCM, Changsha (410007
张进华	YANG Xiao, WEI Yi, LIU Xiaowen, et al. Hunan Medical College of TCM, Changsha (410007
朱文锋	YANG Xiao, WEI Yi, LIU Xiaowen, et al. Hunan Medical College of TCM, Changsha (410007
向华林	YANG Xiao, WEI Yi, LIU Xiaowen, et al. Hunan Medical College of TCM, Changsha (410007

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中文摘要:

目的 :探讨补肾清利活血法延缓肾小球硬化的有效性和作用环节。方法 :在 5/ 6肾切除残肾模型大鼠的基础上 ,运用免疫组织化学及常规病理等方法观察补肾清利活血法对肾小球细胞外基质 (ECM )蓄积及其部分调控机制的影响。结果 :残肾模型大鼠术后 1周开始给予补肾清利活血中药 ,术后 5周血肌酐(SCr)、尿素氮 (BUN)、尿蛋白显著降低 ;病理结果示ECM多种成分 (Ⅰ和Ⅳ型胶原、层粘连蛋白 )的蓄积显著减少 ,肾小球内细胞数及增殖细胞核抗原阳性细胞数显著减少 ,肾小球硬化程度减轻 ;在降低BUN、SCr方面显著优于西药依那普利。结论 :补肾清利活血法能显著减少残肾模型大鼠肾小球ECM蓄积 ,改善肾功能 ,减缓肾小球硬化

英文摘要:

Objective: To study the effect of Bushen Qingli Huoxue (BQH) treatment on glomerular extracellular matrix (ECM) and in retarding glomerular sclerosis in rats and to explore the mechanism and key of regulation. Methods: Animal model was established by 5/6 nephrectomy and the study was carried out by using immune histochemical and pathological methods. Treatment of BQH started 1 week after operation. Results: After being treated with BQH, the serum creatinine (SCr), blood urea nitrogen (BUN) and urinary protein excretion reduced markedly. Pathological examination showed accumulation of ingredients of glomerular ECM such as type Ⅰ, Ⅳ collagen and laminin decreased significantly, the number of glomerular cells and proliferation cell nuclear antigen positive cells also decreased obviously, and showed that the glomerular sclerosis was retarded. The effect of BQH was similar to that of Enapril, but superior to that of Enapril in reducing BUN and SCr. Conclusion: BQH has the effects of reducing ECM accumulation, improving renal function and retarding glomerular sclerosis.

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