甘草提取物诱导胃癌MGC-803细胞凋亡的初步研究

作者	单位
马靖	MA Jing, PENG Wenlie, LIANG Dong, et al. Department of Biochemistry, College of Life Science, Zhongshan University, Guangzhou (510275
彭文烈	MA Jing, PENG Wenlie, LIANG Dong, et al. Department of Biochemistry, College of Life Science, Zhongshan University, Guangzhou (510275
梁东	MA Jing, PENG Wenlie, LIANG Dong, et al. Department of Biochemistry, College of Life Science, Zhongshan University, Guangzhou (510275
庞大本	MA Jing, PENG Wenlie, LIANG Dong, et al. Department of Biochemistry, College of Life Science, Zhongshan University, Guangzhou (510275
徐安龙	MA Jing, PENG Wenlie, LIANG Dong, et al. Department of Biochemistry, College of Life Science, Zhongshan University, Guangzhou (510275

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中文摘要:

目的 :研究甘草提取物诱导胃癌MGC 80 3细胞发生凋亡。方法 :用荧光双染、琼脂糖凝胶电泳、流式细胞仪等方法 ,观察甘草提取物处理MGC 80 3细胞后细胞形态和染色质DNA等的变化 ,以双重免疫标记法和免疫组化法检测处理前后 p53基因表达的变化。结果 :激光扫描共聚焦显微镜观察到处于不同凋亡进程中的细胞 ;流式细胞仪检测到显著的凋亡峰 ,且凋亡百分率呈浓度、时间依赖性 ;高浓度甘草提取物处理的MGC 80 3细胞的DNA琼脂糖凝胶电泳呈现典型的“ladder” ;甘草提取物对p53基因表达没有影响。结论 :甘草通过p53非依赖途径诱导MGC 80 3细胞凋亡 ,可望作为一种新的细胞凋亡诱导剂用于胃癌的治疗。

英文摘要:

To study the apoptosis in human gastric cancer cell MGC-803 induced by the extract of glycyrrhiza uralensis Fisch. Methods: Apoptosis was detected by laser scanning confocal microscope, agarose gel electrophoresis and flow cytometry. The p53 gene expression was analyzed by double immunolabelling and immunohistochemistry technique. Results: Chromatin condensation of different phases of apoptotic cells were observed by laser scanning confocal microscope, the percentage of apoptosis determined by flow cytometry was time- and dose-dependent, agarose gel electrophoresis of DNA exhibited obvious “ladder”in the cells treated with higher concentration of the extract of glycyrrhiza uralensis. The p53 gene expression was unchanged after treatment. Conclusion: Glycyrrhiza can induce human gastric cancer cell line MGC-803 to apoptosis by p53-independent pathway and can be used as a potential, natural apoptosis-inducing agent for gastric cancer therapy.

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