雷公藤多甙对哮喘患者Th<sub>1</sub>、Th<sub>2</sub>细胞因子产生的影响

作者	单位
林科雄	第三军医大学新桥医院呼吸内科研究所!重庆400037
王长征	第三军医大学新桥医院呼吸内科研究所!重庆400037
钱桂生	第三军医大学新桥医院呼吸内科研究所!重庆400037

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中文摘要:

目的 :了解雷公藤多甙对哮喘患者Th1、Th2 细胞因子的影响 ,进一步探讨雷公藤多甙治疗哮喘的机制。方法 :选 12例中、重度哮喘患者 ,口服雷公藤多甙每日 4 0mg或 6 0mg治疗 4周 ,治疗前后取外周血制备血清 ,并取治疗前外周血分离单个核细胞 (PBMC) ,在体外用刀豆蛋白 (ConA)刺激 6h后再加入雷公藤甲素处理 2 4h ,收集培养上清 ,用酶联免疫吸附试验 (ELISA)对患者血清及PBMC培养上清IL 2、IL 4、IL 5、γ干扰素 (IFN γ)水平进行检测。结果 :(1)雷公藤多甙治疗后哮喘患者血清中IL 2、IL 4、IL 5水平较治疗前降低 (P <0 0 1) ,而IFN γ治疗前后均在检测灵敏度 (2 5pg/ml)以下。 (2 )雷公藤甲素处理后哮喘患者PBMC分泌IL 2、IL 4、IL 5减少 (P <0 0 1,P <0 0 5) ,而IFN γ也在检测灵敏度以下。结论 :雷公藤多甙对哮喘患者Th2 细胞因子的产生具有明显的抑制作用 ,是治疗哮喘的重要机制。但雷公藤多甙对Th1细胞因子的产生也有抑制作用 ,说明雷公藤抑制Th2 、Th1细胞因子产生的作用无特异性。

英文摘要:

To observe the effect of Tripterygium wilfordii on Th 1, Th 2 cytokines in asthma patients for further study on the therapeutic mechanism. Methods: Twelve patients of middle or severe asthma were treated by Tripterygium polyglucoside 40 mg or 60 mg daily for 4 weeks. Blood of patients was collected before and after treatment for serum and peripherol blood mononuclear cells (PBMC) preparation. The prepared PBMCs were stimulated in vitro with Concanavalin A (ConA) for 6 hrs and followed by culturing with Triptolide for 24 hrs and then the supernatant was collected. The concentration of interleukin-2(IL-2),-4(IL-4), -5(IL-5) and interferon-γ(IFN-γ) in serum and in the supernatant were detected by enzyme-linked immunosorbent assay (ELISA). Results: Serum levels of IL-2, IL-4 and IL-5 of patients decreased significantly after treatment of Tripterygium polyglucoside (P<0 01), but IFN-γ level was under the detection sensitivity both before and after treatment. Triptolide could inhibit PBMC to secrete IL-2, IL-4 and IL-5 in vitro (P<0 01), but IFN-γ was also under the detection sensitivity. Conclusion: The marked inhibition of Th 2 cytokine expression by Tripterygium was the important mechanism of it in treating asthma. But the fact that Tripterygium also showed inhibition on Th 1 cytokine indicated that the inhibition of Tripterygium on Th 2 and Th 1 cytokines was non-specific.

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