参麦注射液对内毒素所致小鼠全身炎症反应综合征和多器官功能失常综合征保护作用的实验研究

Experimental Study of Protective Effect of Shenmai Injection on Endotoxin Induced Systemic Inflammatory Reaction Syndrome and Multiple Organ Dysfunction Syndrome

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中文关键词: 参麦注射液全身炎症反应综合征多器官功能失常综合征内毒素

英文关键词:Shenmai injection systemic inflammatory reaction syndrome multiple organ dysfunction syndrome endotoxin

基金项目:国家中医药管理局课题! (No .97L0 2 8)

作者	单位
魏育林	北京中日友好医院!北京100029
李亚俊	北京中日友好医院!北京100029
刘轩	北京中日友好医院!北京100029
仇文颖	北京中日友好医院!北京100029
戴书静	北京中日友好医院!北京100029
罗乃丹	北京中日友好医院!北京100029
刘东玉	北京中日友好医院!北京100029
王伟钢	首都医科大学
金恩源	北京中医药大学

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中文摘要:

目的 :应用内毒素 (LPS)所致小鼠全身炎症反应综合征 (SIRS)、多器官功能失常综合征(MODS)动物模型 ,研究参麦注射液对SIRS、MODS的保护作用。方法 :将雄性、5～ 7周龄BALB/c小鼠随机分为正常组、LPS模型组和参麦注射液加LPS组。根据SIRS和MODS临床诊断标准 ,检查小鼠肛温、外周血白细胞计数、血液生化及肺脏和肝脏病理学 ,并测定血浆内毒素和血清肿瘤坏死因子 (TNFα)水平。结果 :腹腔注射LPS可致内毒素血症和高TNFα血症 ;可使小鼠体温明显降低 ,外周血白细胞数减少 ;能明显降低血糖水平 ,增加血清甘油三酯和胆固醇水平。病理结果显示 ,LPS可致肺和肝脏毛细血管扩张、充血、水肿和炎细胞浸润。参麦注射液可明显升高LPS所致小鼠的低体温和低外周血白细胞数 ;可改善内毒素所致小鼠低血糖状态和减轻LPS所致的各脏器病理改变 ;可明显降低血浆内毒素和TNFα水平。结论 :参麦注射液对LPS所致小鼠SIRS和MODS具有显著的保护作用。其保护机制可能与降低内毒素水平和减少TNFα分泌有关。

英文摘要:

To study the protective effect of Shenmai injection (SMI) on systemic inflammatory reaction syndrome (SIRS) and multiple organ dysfunction syndrome (MODS) in animal model of SIRS、MODS induced by lipopolysaccharide (LPS). Methods: Male BALB/c mice, 5～7 weeks old were randomly divided into 3 groups, the normal control group, the LPS model group and the LPS +SMI group. The rectal temperature, peripheral white blood cell count (WBC), blood biochemical examination, histopathologic changes of lung and liver as well as the blood levels of endotoxin and tumor necrosis factor α (TNFα) were determined before and after treatment. Results: LPS could induce endotoxemia and high serum level of TNFα, decrease the rectal temperature and WBC, reduce blood glucose and increase serum triglyceride and cholesterol levels. Pathological examination showed that LPS could cause pulmonary alveolar congestion, edema, exudation, capillary dilation and inflammatory cell infiltration in liver and lung tissue. SMI could significantly raise the low body temperature caused by LPS and reduction of WBC, improve the hypoglycemia and high plasma TNFα level, alleviate the pathologic changes in organs and reduce the plasma level of LPS (endotoxin). Conclusion: SMI has marked effect in protecting LPS caused SIRS、MODS, the mechanism might be related with the lowering of LPS (endotoxin) level and reducing of TNFα secretion.

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