芦荟大黄素对球囊动脉血管损伤后体外培养的平滑肌细胞c-myc基因表达的影响

目的 :探讨芦荟大黄素抑制平滑肌细胞增殖的作用机制。方法 :用 3FFogarty球囊拉栓导管对纯种日本大耳白兔腹主动脉进行球囊内皮剥脱损伤 ,4 8h后取出腹主动脉中膜进行原代平滑肌细胞培养 ,细胞同步于G0 /G1期后 ,实验组加芦荟大黄素 2 0 μg/ml,对照组加等体积的细胞培养液 ,3h后分别用North ern杂交法、Western杂交法和免疫细胞化学技术检测两组动物平滑肌细胞c myc基因mRNA和蛋白表达产物的变化。结果 :与对照组比较 ,实验组平滑肌细胞c mycmRNA和蛋白水平的表达变化差异均无显著性。结论 :芦荟大黄素对平滑肌细胞的增殖抑制作用可能不是通过影响c myc基因的表达实现的 ,而是通过其他途径。

英文摘要:

To study the molecular mechanism of aloe emodin (AE) in inhibiting smooth muscle cells′ (SMC) proliferation. Methods: Deendothelialization was performed in abdominal aorta of Japanese white ear rabbits by using 3F Fogarty arterial embolectomy catheter. The media of abdominal aorta was isolated 48 hrs later for performing primary SMC culture. Cells were synchronized with G 0/G 1 phase by serum starvation, the AE was then added to the culture medium of experimental group, at a concentration of 20 μg/ml, and to the control group, equal volume of culture solution was added instead. The c myc mRNA and protein expressions were examined 3 hrs later by using techniques of Northern blotting, Western blotting and immunocytochemistry respectively. Results: In comparing with the control group, neither the expression of c myc mRNA nor the expression of c myc protein was changed after addition of AE. Conclusion: The inhibition of AE on SMC might not be due to influencing c myc expression, but via other pathway.

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