消瘤平移合剂含药血清诱导肝癌H-7402细胞凋亡的实验研究

目的 :观察消瘤平移合剂含药血清对肝癌H 74 0 2细胞凋亡的诱导作用。方法 :运用血清药理学方法 ,选择不同浓度的含药血清与人肝癌H 74 0 2细胞共同孵育 ,于不同时间观察 ,并与空白血清作对照。结果 :消瘤平移合剂含药血清可显著抑制H 74 0 2细胞的增长 ;H 74 0 2细胞在显微镜下呈现典型的凋亡形态学改变 ,流式细胞仪检测可见亚G1峰 ,凋亡呈含药血清浓度、时间依赖性特点 ,对照组及 5 %、10 %含药血清作用 2 4h ,其凋亡率分别为 0 4 9%、5 5 8%、8 4 6% ,4 8h凋亡率分别为 0 83%、11 5 8%、14 2 5 % ;bcl 2基因检测显示bcl 2基因表达受抑。结论 :消瘤平移合剂可通过诱导肿瘤细胞凋亡 ,发挥其临床抗肿瘤、抗转移的作用。

英文摘要:

Objective: To observe the efficacy of Xiaoliu Pingyi Mixture (XLPY) in inducing the apoptosis of human hepatocellular carcinoma (HCC) cell line H 7402. Methods: Using serum pharmacologic method, the morphological change of apoptotic cells was observed at different time points by co culturing with different concentrations of medicated serum containing XLPY with light and fluorescent microscopy, flow cytometry, and immunohistochemical staining methods. Blank serum was taken as control. Results: The different concentration of medicated serum containing XLPY could significantly inhibit the growth of H 7402 cell line in vitro. After treated with medicated serum of XLPY, the cellular morphologic changes were typical apoptosis under light and fluorescent microscopy, the sub G 1 peaks were observed by flow cytometric assays. The apoptosis rate revealed a medicated serum concentration and time dependent manner. The control group 5%, 10% medicated serum induced apoptotic rate were 0.49%, 5.58% and 8.46% by 24h; while by 48h, it was 0.83%, 11.58% and 14.25% respectively. bcl 2 gene examination showed that the bcl 2 gene expression was inhibited. Conclusion: XLPY could induce apoptosis in carcinoma cells, and play the clinical role of anti tumor and anti metastasis.

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