五子衍宗丸对男性老年肾虚者外周血白细胞线粒体DNA缺失及线粒体呼吸链酶复合体活力的影响

Effect of Wuzi Yanzong Pill on Mitochondrial DNA Deletion and Respirato ry Chain Enzyme Complex Activity in Peripheral Leukocyte of Aged Male with Kidne y Deficiency Syndrome

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DOI：

中文关键词: 五子衍宗丸衰老白细胞线粒体DNA 呼吸链酶复合体

英文关键词:Wuzi Yanzong Pill aging leukocyte mitochondr ial DNA respiratory chain enzyme complex

基金项目:国家自然科学基金资助项目 (No .3980 0 1 99)

作者	单位
王学美	北京大学第一医院中西医结合研究所北京100034
富宏	北京大学第一医院中西医结合研究所北京100034
刘庚信	北京大学第一医院中西医结合研究所北京100034

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中文摘要:

目的 :研究五子衍宗丸对男性老年肾虚者外周血白细胞线粒体DNA缺失、线粒体呼吸链酶复合体活力的影响。方法 :38例男性老年肾虚者采用单盲法 ,随机分为五子衍宗丸治疗组和安慰剂对照组 ,服药 3个月。分别用聚合酶链反应 (PCR)和酶动力学方法检测治疗前后外周血白细胞线粒体DNA缺失及线粒体呼吸链酶复合体的活力。结果 :五子衍宗丸可提高男性老年肾虚者外周血白细胞线粒体呼吸链酶复合体Ⅰ、Ⅳ活力 ,减少线粒体DNA缺失 (P <0 0 5 ,P <0 0 1)。结论 :五子衍宗丸对男性老年肾虚者白细胞线粒体DNA氧化损伤有保护作用

英文摘要:

To investigate the effect of Wuzi Yanzong Pil l (WZYZP) on mitochondrial DNA (mtDNA) deletion and respiratory chain enzyme compl ex (RCZC ) in peripheral blood leukocyte of aged male with Kidney Deficiency Syndrome (KD S). Methods: Single blinded study was conducted in 38 aged ma le with KDS, who were randomly divided into 2 groups treated with WZYZP and plac ebo respe ctively for 3 months. Levels of mtDNA deletion and RCZC were determined by polym erase chain reaction (PCR) and enzyme kinetics technique respectively. Results: WZYZP could reduce the mtDNA deletion and raise the a ctivity of mitochondrial RCZC Ⅰ, Ⅳ in peripheral blood leukocyte of aged male with KDS (P<0 05, P<0 01). Conclusion: WZYZP has protective effect on mtDNA from oxidativ e damage in leukocyte of aged male with KDS.

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