| 郭为民,沈自尹,陈瑜,陈伟华,应健.补肾、活血类复方对老年大鼠T细胞凋亡相关基因表达调控模式的比较研究[J].中国中西医结合杂志,2002,(3):203-206 |
| 补肾、活血类复方对老年大鼠T细胞凋亡相关基因表达调控模式的比较研究 |
| Comparative Study on Regulation Patterns of Compound Prescriptions for Kidney Tonifying and for Blood Circulation Activating on T-cell Apoptosis Related Gene Expression in Aged Rats |
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| DOI： |
| 中文关键词: 补肾复方 活血复方 老年大鼠 激活诱导T细胞凋亡 凋亡相关基因 |
| 英文关键词:compound prescription for Kidney tonifying compound prescription for blood circulation activating old rat activation inducing T cell apoptosis apoptosis related gene |
| 基金项目:国家自然科学基金资助课题 (No .30 0 70 90 7) |
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| 摘要点击次数: 1376 |
| 全文下载次数: 1926 |
| 中文摘要: |
| 目的 :探讨两个补肾复方 (右归饮和补肾益寿胶囊 )及活血复方下调老年大鼠T细胞凋亡的基因调控模式。方法 :用TUNEL标记的流式细胞检测和荧光实时定量RT PCR技术 ,研究老年大鼠和年轻大鼠T淋巴细胞抗凋亡和促凋亡基因 (Fas、FasL、Bcl 2、Bax、TNFR1、TNFR2 )的表达以及凋亡级联反应中半胱氨酸蛋白酶 (如Caspase8和Caspase3)活性的差异 ,及比较两个补肾复方和活血复方对老年大鼠T细胞抗凋亡和促凋亡基因表达的调控以及对Caspase活性的影响。 结果 :两个补肾复方均能够有效地降低老年大鼠T细胞的过度凋亡 ,下调FasL及TNFR1基因的转录和Caspase8及Caspase3的活性。而活血复方对于T细胞的过度凋亡无显著作用。结论 :激活诱导的T细胞过量凋亡与肾虚密切相关 ,两个补肾复方均可下调促凋亡基因FasL和TNFR1的转录 ,从而抑制老年大鼠过度的T细胞凋亡 ,是补肾法所特有的对老年T细胞凋亡相关基因的调控模式。 |
| 英文摘要: |
| Objective: To investigate the regulation pattern of the two compound prescriptions for Kidney tonifying, Yougui Yin and Bushen Yishou capsule, in down regulating T cell apoptosis gene expression in aged rats. Methods: Expressions of T cell apoptosis promoting and inhibiting genes, including Fas, FasL, Bcl 2, Bax, TNFR 1 and TNFR 2, as well as activity of cysteine proteinase in cascade connection, such as Caspase 8 and Caspase 3 were determined by TUNEL labeled flow cytometry and fluorescence real time quantitative RT PCR technique. The difference between old and young rats was compared, and the different regulation patterns of the two compound prescriptions for Kidney tonifying and their effects on Caspase activity were compared with those of compound prescription for blood circulation activating. Results: The two compound prescriptions for Kidney tonifying could effectively lower T cell over apoptosis in old rats, down regulate FasL and TNFR 1 gene transcription, and decrease the activity of Caspase 8 and Caspase 3, while the compound prescription for blood circulation activating showed insignificant effect on T cell over apoptosis. Conclusion: Kidney deficiency is closely related to the T cell over apoptosis. The T cell over apoptosis in old rats could be effectively improved by the two compound prescription for Kidney tonifying through down regulating the apoptosis promoting genes FasL and TNFR 1 transcription. That is the unique regulation pattern of Kidney tonifying principle to T cell apoptosis related gene in old rats. |
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