芦丁抑制家兔血小板激活因子诱导血小板活化作用的实验研究

作者	单位
陈文梅	北京市心肺血管疾病研究所药理研究室北京100029
金鸣	北京市心肺血管疾病研究所药理研究室北京100029
吴伟	北京市心肺血管疾病研究所药理研究室北京100029
李金荣	北京市心肺血管疾病研究所药理研究室北京100029
臧宝霞	北京市心肺血管疾病研究所药理研究室北京100029
朴永哲	北京市心肺血管疾病研究所药理研究室北京100029

摘要点击次数: 1477

全文下载次数: 1735

中文摘要:

目的 :观察芦丁对家兔血小板激活因子 (PAF)诱导血小板聚集、5 羟色胺 (5 HT)释放、血小板内游离钙离子浓度的影响及其机理。方法 :以比浊法测定家兔洗涤血小板 (WRP)聚集率 ,邻苯二甲醛(OPT)荧光分光光度法测定 5 HT含量 ,以Fura 2荧光分光光度法测定血小板内游离钙离子浓度的变化。结果 :芦丁体外呈浓度依赖性抑制PAF(9 5 5× 10 - 9mol/L)诱发的WRP聚集、5 HT释放作用 ,IC50 分别为0 73、1 13mmol/L ;同时 6 8 3、136、2 74、5 45 μmol/L芦丁剂量依赖地抑制PAF(4 78× 10 - 10 mol/L)引起的血小板内游离钙浓度增高。结论 :芦丁可抑制PAF诱导的血小板聚集、5 HT释放及血小板内游离钙浓度增加。

英文摘要:

Objective: To explore the inhibitory effect and mechanism of rutin against platelet activating factor (PAF) induced platelet aggregation, 5 HT release and intra platelet free calcium concentration. Methods: The rate of washed rabbit platelet (WRP) aggregation was measured by turbidimetry and O phthaldialdehyde (OPT) fluoro spectrophotometry (FSPM) was used to determine 5 HT content. The intraplatelet free calcium concentration was measured with Fura 2/AM FSPM assay. Results: Rutin in vitro was concentration dependently inhibiting PAF (9.55×10 -9 mol/L) induced WRP aggregation, the IC 50 of 5 HT release was 0.73, 1.13 mmol/L respectively and the intraplatelet free calcium concentration elevation evoked by PAF (4.78×10 -10 mol/L) were inhibited by 68.3, 136, 274, 545 μmol/L of rutin dose dependently. Conclusion: Rutin could inhibit PAF induced platelet aggregation, 5 HT release and the increase of intraplatelet free calcium.

关闭