人参多糖和当归多糖诱导人内皮细胞表达造血生长因子的实验研究

Experimental Study on Effect of Ginseng Polysaccharide and Angelica Polysaccharide in Inducing Hematopoietic Growth Factors in Human Endothelial Cells

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中文关键词: 人参多糖当归多糖内皮细胞造血祖细胞造血生长因子

英文关键词:Ginseng polysaccharide Angelica polysaccharide endothelial cell hematopoietic progenitors hematopoietic growth factor

基金项目:国家自然科学基金资助课题 (No .39870 91 2 )

作者	单位
吴宏	重庆医科大学基础医学院组胚教研室重庆400016
姜蓉	重庆医科大学基础医学院组胚教研室重庆400016
郑敏	重庆医科大学基础医学院组胚教研室重庆400016
王亚平	重庆医科大学基础医学院组胚教研室重庆400016

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中文摘要:

目的 :研究和论证人参和当归促进血细胞生成及“补气生血”的现代生物学机理。方法 :采用造血生长因子生物活性检测与免疫细胞化学等技术 ,研究经人参多糖 (GPS)和当归多糖 (APS)诱导的人脐静脉内皮细胞株 (Ecv30 4 )上清液 (HEcCM)中造血生长因子的活性及粒单系集落刺激因子 (GM CSF)、IL 3和IL 6在内皮细胞的表达。结果 :GPS和APS体外诱导制备的HEcCM能显著促进粒单系造血祖细胞(CFU GM )和多向性造血祖细胞 (CFU Mix)的增殖 ;同时 ,GPS和APS能不同程度地促进内皮细胞GM CSF、IL 3和IL 6蛋白表达。结论 :GPS和APS能上调造血微环境中的内皮细胞分泌造血生长因子来调节血细胞的生成。

英文摘要:

Objective: To explore the modern biological mechanism of Ginseng and Angelica in replenishing Qi to nourish blood. Methods: Using biological assay of hematopoietic growth factor and immunocytochemical technique, the hematopoietic growth factor activity in conditioned culture media of human umbilical vein endothelial cell (HUVEC) and expression of granulomono colony stimulating factor (GM CSF), interleukin 3 (IL 3) and interleukin 6 (IL 6) in the endothelial cells were determined. Results: Ginseng polysaccharide (GPS) and Angelica polysaccharide (APS) could markedly promote the proliferation and differentiation of colony forming unit granulocyte macrophage (CFU GM) and CFU Mix in the culture media of HUVEC, and enhance the expression of GM CSF, IL 3 and IL 6 in various degrees. Conclusion: GSP and ASP could up regulate the endothelial cells expression to secrete hematopoietic growth factors in the micro environment so as to up regulate the hematopoiesis.

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